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PMID: 7197923 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conversion of isopenicillin N into penicillin N in cell-free extracts of Cephalosporium acremonium.

The Biochemical journal ·Vol. 194 ·No. 2 ·1981-02-15 ·Pages 645-7

Jayatilake GS, Huddleston JA, Abraham EP

Abstract

In a cell-free system prepared by osmotic lysis of protoplasts of Cephalosporium acremonium, isopenicillin N is converted into penicillin N. The epimerase activity of the system is labile.

MeSH Terms

Acremonium/enzymology,metabolism Penicillins/metabolism Racemases and Epimerases/metabolism

Chemicals

Penicillins Racemases and Epimerases penicillin N

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jayatilake G S

Huddleston J A

Abraham E P

References (9)

9 references, click to expand

Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp.
Biochem J. 1967 Jun;103(3):877-90 PMID: 6069166
Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9.
Biochem J. 1974 Oct;143(1):115-27 PMID: 4219278
Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid.
Biochem J. 1975 Dec;151(3):729-39 PMID: 175787
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Purification and some properties of cephalosporin N, a new penicillin.
Biochem J. 1954 Sep;58(1):94-102 PMID: 13198858
Incorporation of 3H from delta-(L-alpha-amino (4,5-3H)adipyl)-L-cysteinyl-D-(4,4-3H)valine into isopenicillin N.
Biochem J. 1979 Nov 15;184(2):421-6 PMID: 575040
Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium.
Biochem J. 1979 Nov 15;184(2):427-30 PMID: 575041
Incorporation of 3H and 14C from (6 alpha-3H)penicillin N and (10-14C,6 alpha-3H) penicillin N into deacetoxycephalosporin C.
Biochem J. 1980 Mar 15;186(3):889-95 PMID: 7190385
Synthesis of delta-(alpha-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis.
Biochem J. 1976 Sep 1;157(3):651-60 PMID: 825110

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1940645

Published

1981-02-15

Pages

645-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1162791

Subset

External Links

PubMed source DOI: 10.1042/bj1940645 PMC Full Text View journal details

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Conversion of isopenicillin N into penicillin N in cell-free extracts of Cephalosporium acremonium.

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