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PMID: 7197672 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Membrane and cytoplasmic nitrate reductase of Staphylococcus aureus and application of crossed immunoelectrophoresis.

Journal of bacteriology ·Vol. 148 ·No. 2 ·1981-11-00 ·Pages 724-7

Burke KA, Brown AE, Lascelles J

Abstract

Specific antiserum to the membrane nitrate reductase of Staphylococcus aureus was derived from immunoprecipitates on crossed immunoelectrophoresis plates. Analysis of the cytoplasmic and membrane forms of the enzyme in cells grown with nitrate and azide indicated their identity, and in each case, the major subunit, Mr 140,000, was converted by trypsin to a polypeptide, Mr 112,000, without loss of enzyme activity or immunological reactivity.

MeSH Terms
Cell Membrane/enzymology Cytoplasm/enzymology Immunoelectrophoresis, Two-Dimensional Nitrate Reductases/analysis,metabolism Staphylococcus aureus/enzymology Trypsin/pharmacology
Chemicals
Nitrate Reductases Trypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burke K A
Brown A E
Lascelles J
References (11)
11 references, click to expand
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  2. Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3711-5 PMID: 127177
  3. Biosynthesis of membrane-bound nitrate reductase in Escherichia coli: evidence for a soluble precursor.
    J Bacteriol. 1976 Apr;126(1):122-31 PMID: 770417
  4. Nitrate reductase activity in heme-deficient mutants of Staphylococcus aureus.
    J Bacteriol. 1976 Apr;126(1):225-31 PMID: 1262303
  5. sn-Glycerol-3-phosphate dehydrogenase and its interaction with nitrate reductase in wild-type and hem mutant strains of Staphylococcus aureus.
    J Bacteriol. 1978 Feb;133(2):621-5 PMID: 637913
  6. Synthesis and degradation of nitrate reductase in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):352-9 PMID: 7012121
  7. Bacterial cytochromes and their spectral characterization.
    Methods Enzymol. 1978;53:202-12 PMID: 213679
  8. Partial purification and some properties of the Staphylococcus aureus cytoplasmic nitrate reductase.
    J Bacteriol. 1979 Jul;139(1):120-5 PMID: 457598
  9. Heme-deficient mutants of Staphylococcus aureus.
    Methods Enzymol. 1979;56:172-8 PMID: 459864
  10. Biosynthesis and membrane binding of succinate dehydrogenase in Bacillus subtilis.
    J Bacteriol. 1980 Dec;144(3):941-51 PMID: 6777371
  11. Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis.
    J Bacteriol. 1978 Oct;136(1):304-11 PMID: 101513
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.2.724-727.1981
Published
1981-11-00
Pages
724-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216261
Subset
IM
Grants
NIADDK NIH HHS · AM-1114 · United States
External Links
PubMed source DOI: 10.1128/jb.148.2.724-727.1981 PMC Full Text View journal details
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