Home LiteratureArticle Details
PMID: 7190148 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Desmosome development in an in vitro model.

The Journal of cell biology ·Vol. 85 ·No. 3 ·1980-06-00 ·Pages 695-702

Dembitzer HM, Herz F, Schermer A, Wolley RC, Koss LG

Abstract

A model has been devised to study the in vitro formation of desmonsomes. The model is based on the differential labeling of two subpopulations of a desmosome-forming human cancer line (C4I). The labeled subpopulations are dispersed, preincubated separately on a shaking water bath for 24 h to allow the internalization of desmosome fragments and the repair of the cell surface, and then mixed, and allowed to aggregate. Aliquots of the mixed suspension are fixed at various intervals. The time between mixing and fixation represents the maximum age of any junction between dissimilarly labeled cells. The beginnings of desmosome formation were observed within a few minutes after the beginning of aggregation. Close apposition of cell membranes was seen immediately after mixing, followed within 15 min by the appearance of a submembrane density in one or both of the interacting cells. Intracytoplasmic filament formation takes place at between 15 and 30 min. Desmosome formation is complete by 90 min. The process is accompanied by a progressive widening of the extracellular space and the desification and organization of the extracellular material and the submembrane plaques.

MeSH Terms
Cell Adhesion Cell Differentiation Cell Line Cell Membrane/ultrastructure Cells, Cultured Cytoskeleton/ultrastructure Desmosomes/ultrastructure Humans Microscopy, Electron Models, Biological
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dembitzer H M
Herz F
Schermer A
Wolley R C
Koss L G
References (60)
60 references, click to expand
  1. Fine structure of desmosomes. , hemidesmosomes, and an adepidermal globular layer in developing newt epidermis.
    J Cell Biol. 1966 Jan;28(1):51-72 PMID: 5901500
  2. Cell contact during early morphogenesis in the chick embryo.
    Dev Biol. 1967 Jul;16(1):78-106 PMID: 6035571
  3. Phagocytosis of latex beads by Acanthamoeba. I. Biochemical properties.
    Biochemistry. 1967 Feb;6(2):485-97 PMID: 4860149
  4. The differentiation of chick embryonic skin. An electron microscopic study with a description of a peculiar epidermal cytoplasmic ultrastructure.
    Exp Cell Res. 1968 Sep;52(1):261-83 PMID: 5692040
  5. Histotypic self-organization by trypsin-dissociated and EDTA-dissociated chick embryo cells.
    Exp Cell Res. 1968 Sep;52(1):71-85 PMID: 4300101
  6. The fat of desmosomes in trypsinized tissue.
    J Exp Zool. 1968 Jun;168(2):203-14 PMID: 5692696
  7. Differentiation of cell junctions in metanephrogenic blastema.
    Exp Cell Res. 1968 Oct;52(2):660-3 PMID: 5683634
  8. [Electron microscopic studies of aggregates from dissociated tumor cells].
    Virchows Arch B Cell Pathol. 1969;2(2):114-24 PMID: 4978621
  9. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines.
    J Natl Cancer Inst. 1969 Jul;43(1):151-73 PMID: 5796380
  10. [Desmosomes in the tissue culture of tumor cells].
    Naturwissenschaften. 1969 Oct;56(10):517-8 PMID: 5362736
  11. Intercellular junctions in the developing neural retina of the chick embryo.
    Z Zellforsch Mikrosk Anat. 1970;104(3):405-18 PMID: 4913389
  12. The occurrence of microvilli during spreading and growth of BHK21-C13 fibroblasts.
    Exp Cell Res. 1970 Jan;59(1):124-36 PMID: 5448185
  13. Electron microscopic analysis of aggregation of embryonic cells: the structure and differentiation of aggregates of neural retina cells.
    Dev Biol. 1970 Sep;23(1):36-61 PMID: 5481967
  14. Studies on aggregation of embryonic cells: initial cell adhesions and the formation of intercellular junctions.
    J Morphol. 1970 Nov;132(3):245-63 PMID: 5485921
  15. Macromolecular glucosamine-containing component of the surface of cultivated mouse cells.
    J Cell Sci. 1970 Sep;7(2):337-55 PMID: 5530566
  16. Differentiation of the junctional complex of surface cells in the developing Fundulus blastoderm.
    J Cell Biol. 1971 Mar;48(3):455-72 PMID: 5545331
  17. [Microcinematographic and electron microscopic observations on cell surfaces and cell contacts in the human carcinoma cell culture line HEp 2].
    Virchows Arch B Cell Pathol. 1971;8(2):143-61 PMID: 4996729
  18. A fine structural study of adhesive cell junctions in heterotypic cell aggregates.
    J Cell Biol. 1970 Oct;47(1):197-210 PMID: 4935336
  19. Regeneration of transplantation antigens on mouse cells.
    Transplant Proc. 1971 Mar;3(1):180-2 PMID: 4106304
  20. Experimental manipulation of desmosome formation.
    J Cell Biol. 1973 Mar;56(3):636-46 PMID: 4569311
  21. Early contacts between fibroblasts. An ultrastructural study.
    Exp Cell Res. 1973 Mar 30;78(1):71-8 PMID: 4570584
  22. Membrane ultrastructure at mammalian intercellular junctions.
    Prog Biophys Mol Biol. 1973;26:45-101 PMID: 4122630
  23. A quantitative assay for intercellular adhesion.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1569-73 PMID: 4351182
  24. Desmosomes and their components after cell dissociation and reaggregation in the presence of cytochalasin B.
    J Exp Zool. 1973 Sep;185(3):341-56 PMID: 4795950
  25. Plasma membrane differentiations of keratinizing cells of the wool follicle. II. Desmosomes.
    J Ultrastruct Res. 1973 Oct;45(1):15-29 PMID: 4750502
  26. Hemidesmosome and desmosome morphogenesis during epidermal wound healing.
    J Ultrastruct Res. 1973 Oct;45(1):93-101 PMID: 4750505
  27. Development of preimplantation rabbit embryos in vivo and in vitro. I. An ultrastructural comparison.
    Dev Biol. 1973 Dec;35(2):262-82 PMID: 4363068
  28. A study on the mechanism of intercellular adhesion. Effects of neuraminidase, calcium, and trypsin on the aggregation of suspended HeLa cells.
    J Cell Biol. 1974 Mar;60(3):641-52 PMID: 4824290
  29. Structure and function of intercellular junctions.
    Int Rev Cytol. 1974;39:191-283 PMID: 4611943
  30. Desmosomes in human seminiferous epithelium. An electron microscopic study.
    Virchows Arch B Cell Pathol. 1974;16(2):181-94 PMID: 4216139
  31. Junctional complexes in the preimplantation rabbit embryo.
    Anat Rec. 1975 Jan;181(1):17-33 PMID: 45878
  32. Hybrid desmosomes in aggregated chick and mouse cells.
    J Exp Zool. 1975 Apr;192(1):33-41 PMID: 1127407
  33. Intercellular junctions in the early human embryonic retina.
    J Ultrastruct Res. 1975 Apr;51(1):69-78 PMID: 1127794
  34. Transformation-induced alterations in fibroblast adhesion: masking by trypsin treatment.
    Exp Cell Res. 1975 Mar 1;91(1):31-5 PMID: 165945
  35. Inhibition of mobility of cell-surface receptors by factors which mediate specific cell-cell interactions.
    Nature. 1975 Jul 31;256(5516):416-7 PMID: 1143346
  36. Freeze-fracture replication of junctionsl complexes in unincubated and incubated chick embryos.
    Cell Tissue Res. 1975 Sep 17;162(2):235-52 PMID: 1237352
  37. The major cell surface glycoprotein of chick embryo fibroblasts is an agglutinin.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3158-62 PMID: 1059102
  38. Experiments with junctions of the adhaerens type.
    Curr Top Dev Biol. 1975;10:1-34 PMID: 1104272
  39. The preimplantation mammalian embryo: characterization of intercellular junctions and their appearance during development.
    Dev Biol. 1975 Aug;45(2):231-50 PMID: 1193296
  40. Mating in Chlamydomonas: a system for the study of specific cell adhesion. II. A radioactive flagella-binding assay for quantitation of adhesion.
    J Cell Biol. 1976 Jan;68(1):70-9 PMID: 1245546
  41. Mechanisms of adhesion among cells from neural tissues of the chick embryo.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):577-81 PMID: 54921
  42. Molecular changes in cell surface membranes resulting from trypsinization of sarcoma 180 tumor cells.
    Biochim Biophys Acta. 1976 Apr 5;426(4):630-7 PMID: 1259987
  43. Microvilli and blebs as sources of reserve surface membrane during cell spreading.
    Exp Cell Res. 1976 May;99(2):375-84 PMID: 1269533
  44. The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface.
    Br J Cancer. 1976 Oct;34(4):426-36 PMID: 184812
  45. Transformation-induced alterations in adhesion. Binding of pre-formed cell aggregates to cell layers.
    Exp Cell Res. 1976 Dec;103(2):311-20 PMID: 826401
  46. Formation of junctions and cell sorting in aggregates of chick and mouse cells.
    Dev Biol. 1977 Jan;55(1):103-16 PMID: 832767
  47. Mechanisms of cell adhesion: early-forming junctions between aggregating fibroblasts.
    J Cell Sci. 1976 Dec;22(3):671-84 PMID: 1018053
  48. Chromosome analysis and alkaline phosphatase of C41, a cell line of human cervical origin distinct from HeLa.
    Cancer Res. 1977 Sep;37(9):3209-13 PMID: 560251
  49. The adhension of Chinese hamster cells. I. Effects of temperature, metabolic inhibitors and proteolytic dissection of cell surface macromolecules.
    J Cell Physiol. 1977 Aug;92(2):209-20 PMID: 881433
  50. Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding.
    J Biol Chem. 1977 Oct 10;252(19):6835-40 PMID: 893445
  51. A requirement for trypsin-sensitive cell-surface components for cell-cell interactions of embryonic neural retina cells.
    J Cell Biol. 1977 Oct;75(1):56-66 PMID: 562349
  52. Characterization of intercellular junctions in the preimplantation mouse embryo by freeze-fracture and thin-section electron microscopy.
    Dev Biol. 1977 Dec;61(2):252-61 PMID: 590626
  53. Modified desmosomes in cultured epithelial cells.
    Cytobiologie. 1978 Dec;18(2):360-73 PMID: 569606
  54. The terminal web. A reevaluation of its structure and function.
    J Cell Biol. 1979 Apr;81(1):67-82 PMID: 573268
  55. Observations on cell adhesion and separation following enzyme treatment.
    Exp Cell Res. 1966 Mar;41(3):601-8 PMID: 4952046
  56. Improvements in epoxy resin embedding methods.
    J Biophys Biochem Cytol. 1961 Feb;9:409-14 PMID: 13764136
  57. Desmosome development in normal and reassociating cells in the early chick blastoderm.
    Dev Biol. 1962 Jun;4:532-48 PMID: 14482867
  58. PARTICLE UPTAKE BY POLYMORPHONUCLEAR LEUCOCYTES AND EHRLICH ASCITES-CARCINOMA CELLS.
    Biochem J. 1963 Oct;89:150-6 PMID: 14097356
  59. AN ELECTRON MICROSCOPE STUDY OF DEVELOPING BILE CANALICULI IN THE RAT.
    Anat Rec. 1965 Apr;151:507-29 PMID: 14326981
  60. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix.
    J Natl Cancer Inst. 1962 Mar;28:605-27 PMID: 13863218
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.85.3.695
Published
1980-06-00
Pages
695-702
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111458
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.85.3.695 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2