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PMID: 7189754 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.

The Journal of cell biology ·Vol. 84 ·No. 2 ·1980-02-00 ·Pages 235-45

Murphy DB

Abstract

Meiotic spindles isolated from surf clam oocytes to morphological purity are biochemically complex, consisting of many polypeptides. These proteins fall into two classes: (a) polypeptides that are apparently cytoplasmic proteins and are not specifically associated with the spindle; and (b) polypeptides that are specifically associated with the spindle. A subset of the spindle-associated proteins, including a 250,000 mol wt component, remain with spindle tubulin through cycles of cold depolymerization and warm polymerization, showing that they are microtubule-associated proteins.

MeSH Terms
Animals Bivalvia Electrophoresis, Polyacrylamide Gel Female Glycerol Glycols Meiosis Microtubules/analysis Nucleoproteins/analysis Oocytes/ultrastructure Organoids/ultrastructure Ovum/ultrastructure Peptides/analysis Tubulin/analysis
Chemicals
Glycols Nucleoproteins Peptides Tubulin Glycerol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Murphy D B
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21 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.84.2.235
Published
1980-02-00
Pages
235-45
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110549
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.84.2.235 PMC Full Text View journal details
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