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PMID: 7177776 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Vascular smooth muscle: the first recorded Ca2+ transients.

Pflugers Archiv : European journal of physiology ·Vol. 395 ·No. 1 ·1982-10-00 ·Pages 75-7

Morgan JP, Morgan KG

Abstract

The bioluminescent calcium indicator aequorin was successfully loaded into vascular smooth muscle cells of Amphiuma tridactylum by either microinjection or a new method which makes the cells reversibly hyperpermeable. Both gave similar results; however, the latter method produced larger signals. Vasoconstrictors produced a sustained contraction and a light (calcium) response consisting of two components: a large transient followed by a smaller, sustained response. Electrical stimulation produced a light transient that was much briefer than the contraction. These results suggest that tension can be maintained in smooth muscle in the presence of lower calcium levels than those present during force development.

MeSH Terms
Aequorin Animals Calcium/metabolism Cell Membrane Permeability Electric Stimulation Microinjections Muscle, Smooth, Vascular/metabolism Urodela
Chemicals
Aequorin Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morgan J P
Morgan K G
References (11)
11 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00584972
Published
1982-10-00
Pages
75-7
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
NHLBI NIH HHS · HL 07111 · United States
NHLBI NIH HHS · HL 12186 · United States
NHLBI NIH HHS · HL 27847 · United States
External Links
PubMed source DOI: 10.1007/BF00584972 View journal details
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