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PMID: 7174362 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Ultracytochemistry of aminopeptidase A (angiotensinase A) in the kidney glomerulus and juxtaglomerular apparatus.

Histochemistry ·Vol. 74 ·No. 2 ·1982-00-00 ·Pages 199-212

Kugler P

Abstract

Ultracytochemical studies of the Aminopeptidase A (APA; angiotensinase A; E.C. 3.4.11.7) were performed on perfusion fixed rat and mouse kidneys (low concentration aldehydes) using simultaneous azo coupling with alpha-L-Glu-MNA as substrate and HPR as well as HNF as coupling agents. The studies of glomeruli and juxtaglomerular apparatus (JGA) show that APA is localized mainly at the cell membranes of podocytes and endothelial cells (rat and mouse) and of epitheloid cells (mouse) and Goormaghtigh's cells (rat). Increased APA activities are found in the region of cell contacts of epitheloid cells (mouse) and Goormaghtigh's cells (rat). In the epitheloid cells of mice, reaction product is also observed intracellularly in lysosomal structures. Concerning the functional significance of APA in the glomerulus and JGA, it would appear that this enzyme modifies or regulates angiotensin effects in the glomerulus and JGA through the angiotensin degradation.

MeSH Terms
Adrenalectomy Aminopeptidases/metabolism Animals Female Glutamyl Aminopeptidase Histocytochemistry Juxtaglomerular Apparatus/enzymology,ultrastructure Kidney Glomerulus/enzymology,ultrastructure Male Mice Rats Rats, Inbred Strains
Chemicals
Aminopeptidases Glutamyl Aminopeptidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kugler P
References (46)
46 references, click to expand
  1. Localization of aminopeptidase A (angiotensinase A) in the rat and mouse kidney.
    Histochemistry. 1981;72(2):269-78 PMID: 6115833
  2. RENIN-ANGIOTENSIN SYSTEM, ALDOSTERONE, AND SODIUM BALANCE.
    Recent Prog Horm Res. 1965;21:119-77 PMID: 14321056
  3. [Morphology of juxtaglomerular apparatus].
    Verh Anat Ges. 1972;67:109-36 PMID: 4670523
  4. Feedback regulation of nephron filtration rate during pharmacologic interference with the renin-angiotensin and adrenergic systems in rats.
    Kidney Int. 1979 May;15(5):473-86 PMID: 39187
  5. Study on aminopeptidase A.
    Histochemistry. 1980;67(3):267-90 PMID: 6772609
  6. Stimulation of proximal tubular sodium reabsorption by ile5 angiotensin II in the rat kidney.
    Pflugers Arch. 1979 Jul;381(1):83-85 PMID: 573458
  7. Angiotensin II in epitheloid (renin containing) cells of rat kidney.
    Histochemistry. 1981;72(4):499-509 PMID: 6170618
  8. Immunocytochemical demonstration of renin in the mouse and rat kidney after adrenalectomy.
    Histochemistry. 1981;72(3):453-7 PMID: 7028689
  9. Purification of aminopeptidase A in human serum and degradation of angiotensin II by the purified enzyme.
    Biochim Biophys Acta. 1970 Feb 11;198(2):255-70 PMID: 4313532
  10. Renin and angiotensin-like activity in renal lymph.
    J Physiol. 1962 Mar;160:548-63 PMID: 14464509
  11. ARYLAMIDASE ACTIVITY RELATED TO ANGIOTENSINASE.
    Biochim Biophys Acta. 1965 May 18;99:316-24 PMID: 14336068
  12. A mammalian peptidase specific for the hydrolysis of N-terminal alpha-L-glutamyl and aspartyl residues.
    Nature. 1962 Jun 2;194:867 PMID: 13899213
  13. Modulation by prostaglandin synthesis inhibitors of the action of exogenous angiotensin II on glomerular ultrafiltration in the rat.
    Circ Res. 1978 Dec;43(6):889-98 PMID: 709751
  14. [Structure and innervation of the juxtaglomerular apparatus of the rat (author's transl)].
    Adv Anat Embryol Cell Biol. 1978;54(2):3-83 PMID: 655000
  15. Angiotensin II: rapid localization in nuclei of smooth and cardiac muscle.
    Science. 1971 Jun 11;172(3988):1138-9 PMID: 4324923
  16. A method for the study of mechanisms of drug disposition in smooth muscle.
    Can J Physiol Pharmacol. 1968 Sep;46(5):719-30 PMID: 5693070
  17. Effect of angiotensin on the filtration of protein in the rat kidney: a micropuncture study.
    Kidney Int. 1975 Aug;8(2):80-7 PMID: 1160230
  18. High affinity binding of 125I-angiotensin II to rat glomerular basement membranes.
    J Clin Invest. 1977 Jan;59(1):69-81 PMID: 187623
  19. Angiotensin II: renal localization in glomerular mesangial cells by autoradiography.
    Kidney Int. 1975 Oct;8(4):245-54 PMID: 1104968
  20. Angiotensin II binding to renal glomeruli from sodium-loaded and sodium-depleted rats.
    Am J Physiol. 1976 May;230(5):1187-93 PMID: 179329
  21. Dose-dependent stimulation and inhibition of proximal tubular sodium reabsorption by angiotensin II in the rat kidney.
    Pflugers Arch. 1977 Jan 17;367(3):295-7 PMID: 556854
  22. [Significance of juxtaglomerular apparatus for the function of the nephron].
    Verh Anat Ges. 1972;67:137-50 PMID: 4363834
  23. [Quenching of tissue in liquid nitrogen-propane].
    Histochemie. 1970;23 (1):44-50 PMID: 4916959
  24. Renin, a physiologic regulator of renal hemodynamics?
    Circ Res. 1962 Jul;11:185-93 PMID: 14039025
  25. Immunocytochemistry of the renin-angiotensin system: renin, angiotensinogen, angiotensin I, angiotensin II, and converting enzyme in the kidneys of mice, rats, and tree shrews.
    Kidney Int Suppl. 1982 Aug;12:S33-43 PMID: 6290746
  26. Evidence for renal glomerular receptors for angiotensin II.
    Kidney Int. 1974 Oct;6(4):241-6 PMID: 4372450
  27. The intrarenal renin-angiotensin-system. An immunocytochemical study on the localization of renin, angiotensinogen, converting enzyme and the angiotensins in the kidney of mouse and rat.
    Klin Wochenschr. 1982 Oct 1;60(19):1218-22 PMID: 6292570
  28. Angiotensin II immunoreactivity coexists with renin in the juxtaglomerular granular cells of the kidney.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3897-900 PMID: 7022457
  29. [Effects of exogenous angiotensin on the capillaires of cortical and juxta-medullary glomeruli of the rat: study with scanning electron microscopy].
    C R Acad Sci Hebd Seances Acad Sci D. 1971 Sep 27;273(13):1129-31 PMID: 4331280
  30. Angiotensin II effects upon the glomerular microcirculation and ultrafiltration coefficient of the rat.
    J Clin Invest. 1976 Feb;57(2):419-34 PMID: 1254727
  31. Tissue hormones: angiotensin, bradykinin and the regulation of regional blood flows.
    Med Clin North Am. 1968 Mar;52(2):263-81 PMID: 4297806
  32. Bilateral adrenalectomy and the renin-angiotensin system.
    Acta Physiol Acad Sci Hung. 1971;39(4):335-41 PMID: 4107514
  33. The effect of exogenous angiotensin on superficial and deep glomeruli in the rat kidney.
    Kidney Int. 1972 Dec;2(6):336-43 PMID: 4365819
  34. Aminopeptidase A is angiotensinase A. II. Biochemical studies on aminopeptidase A and M in rat kidney homogenate.
    Histochemistry. 1982;74(2):247-61 PMID: 6129208
  35. [The epitheloid cells of the juxtaglomerular apparatus of the guinea pig kidney before and after bilateral adrenalectomy].
    Verh Anat Ges. 1966;61:243-9 PMID: 6001115
  36. Modification of angiotensin-mediated tubulo-glomerular feedback by extracellular volume.
    Kidney Int Suppl. 1975 Sep;:S202-7 PMID: 539
  37. Aminopeptidase A is angiotensinase A- I. Quantitative histochemical studies in the kidney glomerulus.
    Histochemistry. 1982;74(2):229-45 PMID: 7174363
  38. Renin and renal autoregulation.
    Lancet. 1968 Aug 10;2(7563):329-33 PMID: 4173741
  39. RENAL HEMODYNAMICS.
    Am J Med. 1964 May;36:698-719 PMID: 14141449
  40. Renin, renin substrate, and angiotensin I-converting enzyme in the lymph of rats.
    Am J Physiol. 1971 Feb;220(2):307-11 PMID: 4322342
  41. [Influence of the adrenals on the hypertensive effects of renin, and on pressor substances in the kidneys].
    Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1957;230(3):274-83 PMID: 13430841
  42. Ultrastructural injury of arterial endothelium. II. Effects of vasoactive amines.
    Arch Pathol. 1969 Aug;88(2):106-12 PMID: 4307433
  43. BIO-ASSAY OF CIRCULATING RENIN-LIKE PRESSOR MATERIAL BY ISOVOLEMIC CROSS CIRCULATION.
    Am J Physiol. 1963 Aug;205:303-6 PMID: 14058105
  44. Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin.
    J Clin Invest. 1980 Mar;65(3):754-60 PMID: 6153393
  45. The glomerulus, passive filter or regulatory organ?
    Klin Wochenschr. 1980 Oct 1;58(19):957-64 PMID: 7005528
  46. Secretory activity of the juxtaglomerular granular cells of the mouse. Morphologic and enzyme histochemical observations.
    Lab Invest. 1966 Sep;15(9):1459-76 PMID: 5919954
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00495830
Published
1982-00-00
Pages
199-212
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00495830
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