Gas-liquid chromatography technique for detection of hippurate hydrolysis and conversion of fumarate to succinate by microorganisms.

Journal of clinical microbiology ·Vol. 16 ·No. 5 ·1982-11-00 ·Pages 962-4

Kodaka H, Lombard GL, Dowell VR

Abstract

A gas-liquid chromatography technique which allows simultaneous detection of hippuric acid (N-benzoylglycine) hydrolysis and conversion of fumaric acid to succinic acid by microorganisms uses a new medium, hippurate-formate-fumarate broth, and a gas chromatograph equipped with a thermal conductivity detector. This technique gave more reproducible results than other tests used in the study for detecting hippurate hydrolysis and also gave consistent results in detecting succinic acid produced from utilization of fumaric acid.

MeSH Terms

Bacteria/metabolism Chromatography, Gas/methods Culture Media Fumarates/metabolism Hippurates/metabolism Hydrolysis Succinates/metabolism Succinic Acid

Chemicals

Culture Media Fumarates Hippurates Succinates Succinic Acid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kodaka H

Lombard G L

Dowell V R

References (7)

7 references, click to expand

Hippurate hydrolysis as a taxonomic criterion in the genus Streptomyces (order Actinomycetales).
Z Allg Mikrobiol. 1973;13(3):265-72 PMID: 4750185
Rapid hippurate hydrolysis method for presumptive identification of group B streptococci.
J Clin Microbiol. 1975 Jan;1(1):114-5 PMID: 1100648
Rapid, colorimetric test for the determination of hippurate hydrolysis by group B Streptococcus.
J Clin Microbiol. 1976 Jan;3(1):49-50 PMID: 767358
Clinical isolates of anaerobic gram-negative rods with a formate-fumarate energy metabolism: Bacteroides corrodens, Vibrio succionogenes, and unidentified strains.
J Clin Microbiol. 1976 Apr;3(4):432-7 PMID: 770499
Hippurate hydrolysis by Campylobacter fetus.
J Clin Microbiol. 1980 Apr;11(4):435-7 PMID: 7372805
Cultural and physiological characteristics and antimicrobial susceptibility of Clostridium botulinum isolates from foodborne and infant botulism cases.
J Clin Microbiol. 1980 Jun;11(6):604-9 PMID: 7000811
Hippurate hydrolysis by and triphenyltetrazolium tolerance of Campylobacter fetus.
J Clin Microbiol. 1982 Jan;15(1):137-40 PMID: 7186902

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.16.5.962-964.1982

Published

1982-11-00

Pages

962-4

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC272511

Subset

External Links

PubMed source DOI: 10.1128/jcm.16.5.962-964.1982 PMC Full Text View journal details

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Gas-liquid chromatography technique for detection of hippurate hydrolysis and conversion of fumarate to succinate by microorganisms.

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