A macromolecular inhibitor of the antizyme to ornithine decarboxylase.

The Biochemical journal ·Vol. 204 ·No. 3 ·1982-06-15 ·Pages 647-52

Fujita K, Murakami Y, Hayashi S

Abstract

A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, 'antizyme inhibitor', was heat-labile, non diffusable and of similar molecular size to ODC. The antizyme inhibitor re-activated ODC that had been inactivated by antizyme, apparently by replacing ODC in a complex with antizyme. Therefore the antizyme inhibitor can be used to assay the amount of inactive ODC-antizyme complex formed in vitro. When assayed by this method, the complex was shown to be eluted before ODC from a Sephadex G-100 column. Significant increase in ODC activity was observed when the antizyme inhibitor was added to crude liver extracts from rats that had been injected with 1,3-diaminopropane to cause decay of ODC activity, suggesting the presence of inactive ODC-antizyme complex in the extracts.

MeSH Terms

Animals Carboxy-Lyases/antagonists & inhibitors Chromatography, Gel Enzyme Activation/drug effects Kinetics Liver/metabolism Male Ornithine Decarboxylase Inhibitors Proteins/antagonists & inhibitors Rats Rats, Inbred Strains

Chemicals

Ornithine Decarboxylase Inhibitors Proteins ornithine decarboxylase antizyme Carboxy-Lyases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fujita K

Murakami Y

Hayashi S

References (19)

19 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2040647

Published

1982-06-15

Pages

647-52

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1158403

Subset

External Links

PubMed source DOI: 10.1042/bj2040647 PMC Full Text View journal details

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A macromolecular inhibitor of the antizyme to ornithine decarboxylase.

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