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PMID: 7118211 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The complete amino acid sequence of the murine transplantation antigen H-2Db as deduced by molecular cloning.

Immunogenetics ·Vol. 16 ·No. 1 ·1982-00-00 ·Pages 1-9

Reyes AA, Schöld M, Wallace RB

Abstract

A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3'-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3'-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3'-coding ends.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Genes H-2 Antigens/genetics Mice
Chemicals
H-2 Antigens DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reyes A A
Schöld M
Wallace R B
References (31)
31 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00364437
Published
1982-00-00
Pages
1-9
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Grants
NCI NIH HHS · CA16434 · United States
NIGMS NIH HHS · GM26391 · United States
Databases
GENBANK
K00129, M12185
External Links
PubMed source DOI: 10.1007/BF00364437 View journal details
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