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PMID: 7097590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effects of stimulation rate on calcium-dependent action potentials recorded from chick embryo heart cell aggregates.

The Journal of physiology ·Vol. 324 ·1982-03-00 ·Pages 1-10

Mackenzie E, Standen NB

Abstract

1. Action potentials were recorded from aggregates of heart cells prepared from 3- to 7-day chick embryos. At 3 days the maximum rate of rise (+ Vmax) was insensitive to TTX; at 7 days it was considerably reduced by TTX. 2. In the presence of TTX the action potential overshoot was dependent on [Ca]0; the results may be fitted using constant field theory and assuming that the membrane is over a hundred times more permeable to Ca than to Na or K. 3. An increase in stimulation rate in the range 0.2-2 Hz led to an increase in both overshoot and + Vmax. This effect was not seen after addition of 20 mM-tetraethylammonium ions, nor when Sr was substituted for Ca in the external medium. We suggest that these rate-dependent changes may result from partial inactivation of an outward K current.

MeSH Terms
Action Potentials/drug effects Animals Calcium/pharmacology,physiology Cell Aggregation Cells, Cultured Chick Embryo Heart/drug effects,embryology,physiology Kinetics Myocardium/cytology Stimulation, Chemical Strontium/pharmacology Tetraethylammonium Compounds/pharmacology Tetrodotoxin/pharmacology
Chemicals
Tetraethylammonium Compounds Tetrodotoxin Calcium Strontium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mackenzie E
Standen N B
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32 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1982.sp014096
Published
1982-03-00
Pages
1-10
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1250689
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1982.sp014096 PMC Full Text View journal details
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