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PMID: 7088680 Published · ppublish English Journal Article

Applicability of available ion-selective liquid-membrane microelectrodes to intracellular ion-activity measurements.

Pflugers Archiv : European journal of physiology ·Vol. 393 ·No. 1 ·1982-03-00 ·Pages 23-30

Meier PC, Lanter F, Ammann D, Steiner RA, Simon W

Abstract

The equations that govern the behavior of ion-selective electrodes are evaluated for the composition ranges of Li+, Na+, K+, Mg2+, Ca2+, Cl-, and HCO-3 encountered in living cells. Various cause-effect relations are detailed, especially those pertaining to calibration procedures. The relation of the accuracy of measurement to the expected signal range, particularly in connection with the uncertainty of interpolation, is emphasized. Relevant numerical parameters are given for all ISE's discussed, the selectivity characteristics being those of the most advanced membrane systems. Figures are provided for the calibration curves in the vicinity of the typical cytosol composition.

MeSH Terms
Animals Body Fluids/physiology Intracellular Fluid/physiology Ions/physiology Microelectrodes Models, Biological
Chemicals
Ions
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Meier P C
Lanter F
Ammann D
Steiner R A
Simon W
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23 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00582386
Published
1982-03-00
Pages
23-30
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00582386 View journal details
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