Home LiteratureArticle Details
PMID: 7068540 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cadmium and manganese transport in Staphylococcus aureus membrane vesicles.

Journal of bacteriology ·Vol. 150 ·No. 2 ·1982-05-00 ·Pages 973-6

Perry RD, Silver S

Abstract

The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ and Cd2+ were competitive inhibitors of each other's transport, with Km's of 0.95 microM Mn2+ and 0.2 microM Cd2+. The kinetic parameters were nearly identical with vesicles prepared from sensitive and resistant cells, indicating that the cadA-encoded Cd2+ efflux system was inoperative in membrane vesicle preparations. Experiments with energy-inhibited cells indicated that the cadB gene product may bind Cd2+.

MeSH Terms
Biological Transport, Active Cadmium/metabolism,pharmacology Cell Fractionation Cell Membrane/metabolism Kinetics Manganese/metabolism R Factors Staphylococcus aureus/drug effects,metabolism
Chemicals
Cadmium Manganese
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Perry R D
Silver S
References (19)
19 references, click to expand
  1. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus.
    J Bacteriol. 1968 Apr;95(4):1335-42 PMID: 5646621
  2. Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids.
    J Biol Chem. 1971 Oct 10;246(19):5857-61 PMID: 4331061
  3. Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus.
    J Bacteriol. 1972 Nov;112(2):761-72 PMID: 4343823
  4. Mechanisms of active transport in isolated bacterial membrane vesicles. IX. The kinetics and specificity of amino acid transport in Staphylococcus aureus membrane vesicles.
    J Biol Chem. 1972 Dec 10;247(23):7452-8 PMID: 4636316
  5. Mechanism of plasmic-mediated resistance to cadmium in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1975 Jan;7(1):8-14 PMID: 1137361
  6. ATP-linked calcium transport in cells and membrane vesicles of Streptococcus faecalis.
    J Biol Chem. 1978 Apr 10;253(7):2085-92 PMID: 416023
  7. Cation transport alteration associated with plasmid-determined resistance to cadmium in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1978 Dec;14(6):856-65 PMID: 742873
  8. Cation/proton antiport systems in Escherichia coli. Properties of the calcium/proton antiporter.
    J Biol Chem. 1979 Mar 25;254(6):1957-63 PMID: 33991
  9. Cation/proton antiport systems in escherichia coli: properties of the sodium/proton antiporter.
    Arch Biochem Biophys. 1979 Apr 15;194(1):208-14 PMID: 36033
  10. Preparation of everted membrane vesicles from Escherichia coli for the measurement of calcium transport.
    Methods Enzymol. 1979;56:233-41 PMID: 156865
  11. Prokaryotic metallothionein: preliminary characterization of a blue-green alga heavy metal-binding protein.
    Biochem Biophys Res Commun. 1979 Jul 12;89(1):36-43 PMID: 113007
  12. Copper metallothionein, a copper-binding protein from Neurospora crassa.
    Nature. 1980 Mar 27;284(5754):368-70 PMID: 6444697
  13. ATP-linked sodium transport in Streptococcus faecalis. II. Energy coupling in everted membrane vesicles.
    J Biol Chem. 1980 Dec 10;255(23):11403-7 PMID: 6777379
  14. Reduced cadmium transport determined by a resistance plasmid in Staphylococcus aureus.
    J Bacteriol. 1981 Aug;147(2):305-12 PMID: 7263608
  15. Energy-dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureus.
    J Bacteriol. 1981 Aug;147(2):313-9 PMID: 7263609
  16. Mode of action of pamamycin in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1981 Oct;20(4):443-54 PMID: 6177281
  17. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  18. Staphylococci and their classification.
    J Gen Microbiol. 1951 Nov;5(5 Suppl.):1010-23 PMID: 14908038
  19. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS.
    J Gen Microbiol. 1963 Oct;33:121-36 PMID: 14072829
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.150.2.973-976.1982
Published
1982-05-00
Pages
973-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216454
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.150.2.973-976.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2