A modified plaque assay method for accurate analysis of infectivity of influenza viruses with uncleaved hemagglutinin.
A modified plaque assay method for evaluation of infectivity of influenza viruses is described. The modification consists of a double agar overlay, trypsin being included in the second layer added 24 hours after infection. The modified method allows one to determine accurately the infectivity titer of influenza viruses possessing uncleaved hemagglutinin, and to compare the infectivity of viruses possessing cleaved or uncleaved forms of hemagglutinin. The standard method in which trypsin is included into agar overlay just after infection artificially increases the infectivity titer of viruses with uncleaved hemagglutinin and therefore is not suitable for these purposes.
16 references, click to expand
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.Virology. 1974 Feb;57(2):475-90 PMID: 4361457
-
Activation of influenza A viruses by trypsin treatment.Virology. 1975 Dec;68(2):426-39 PMID: 173078
-
The function of the neuraminidase in membrane fusion induced by myxoviruses.Virology. 1980 Dec;107(2):313-9 PMID: 7456332
-
Plaque formation by influenza viruses in the presence of trypsin.J Gen Virol. 1974 Dec;25(3):351-7 PMID: 4475092
-
Fusion between cell membrane and liposomes containing the glycoproteins of influenza virus.Virology. 1980 Jul 30;104(2):294-302 PMID: 7395107
-
Influenza virus-specific T cell-mediated cytotoxicity: integration of the virus antigen into the target cell membrane is essential for target cell formation.Eur J Immunol. 1979 Feb;9(2):107-11 PMID: 312203
-
Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus.Virology. 1971 Jul;45(1):275-88 PMID: 5165251
-
Genetic recombination for antigenic markers of antigenically different strains of influenza B virus.J Virol. 1974 Feb;13(2):347-52 PMID: 16789141
-
Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells.J Virol. 1973 Dec;12(6):1457-65 PMID: 4357516
-
Effect of pancreatin on plaque formation by influenza viruses.Arch Gesamte Virusforsch. 1968;23(4):346-52 PMID: 5680977
-
Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus.Virology. 1976 Jul 15;72(2):494-508 PMID: 948870
-
Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptide.Virology. 1975 Dec;68(2):440-54 PMID: 128196
-
Molecular events during the interaction of envelopes of myxo- and RNA-tumor viruses with cell membranes. A 270 MHz H nuclear magnetic resonance study.Biochim Biophys Acta. 1978 Jul 20;511(1):83-92 PMID: 208610
-
Proteolytic cleavage by plasmin of the HA polypeptide of influenza virus: host cell activation of serum plasminogen.Virology. 1973 Nov;56(1):172-80 PMID: 4795670
-
Studies on the primary structure of the influenza virus hemagglutinin.Proc Natl Acad Sci U S A. 1975 Jan;72(1):93-7 PMID: 1054518
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