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PMID: 7061832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society ·Vol. 30 ·No. 4 ·1982-04-00 ·Pages 401-2

Pette D, Reichmann H

Abstract

A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.

MeSH Terms
Animals Enzymes/isolation & purification Methods Mice Muscles/analysis,enzymology
Chemicals
Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pette D
Reichmann H
Article Info
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Abbr.
J Histochem Cytochem
ISSN
0022-1554
DOI
10.1177/30.4.7061832
Published
1982-04-00
Pages
401-2
Language
English
Region
United States
NLM ID
9815334
Subset
IM
External Links
PubMed source DOI: 10.1177/30.4.7061832 View journal details
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