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PMID: 7057161 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Voltage-dependent conductances in Limulus ventral photoreceptors.

The Journal of general physiology ·Vol. 79 ·No. 2 ·1982-02-00 ·Pages 187-209

Lisman JE, Fain GL, O'Day PM

Abstract

The voltage-dependent conductances of Limulus ventral photoreceptors have been investigated using a voltage-clamp technique. Depolarization in the dark induces inward and outward currents. The inward current is reduced by removing Na+ or Ca2+ and is abolished by removing both ions. These results suggest that both Na+ and Ca2+ carry voltage-dependent inward current. Inward current is insensitive to tetrodotoxin but is blocked by external Ni2+. The outward current has a large transient component that is followed by a smaller maintained component. Intracellular tetraethylammonium preferentially reduces the maintained component, and extracellular 4-amino pyridine preferentially reduces the transient component. Neither component is strongly affected by removal of extracellular Ca2+ or by intracellular injection of EGTA. It is concluded that the photoreceptors contain at least three separate voltage-dependent conductances: 1) a conductance giving rise to inward currents; 2) a delayed rectifier giving rise to maintained outward K+ current; and 3) a rapidly inactivating K+ conductance similar to the A current of molluscan neurons.

MeSH Terms
4-Aminopyridine Aminopyridines/pharmacology Animals Calcium/physiology Electric Conductivity Horseshoe Crabs/physiology Photoreceptor Cells/physiology Sodium/physiology Tetraethylammonium Tetraethylammonium Compounds/pharmacology
Chemicals
Aminopyridines Tetraethylammonium Compounds Tetraethylammonium Sodium 4-Aminopyridine Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lisman J E
Fain G L
O'Day P M
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35 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.79.2.187
Published
1982-02-00
Pages
187-209
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215495
Subset
IM
Grants
NEI NIH HHS · EYO1496 · United States
NEI NIH HHS · EYO1844 · United States
External Links
PubMed source DOI: 10.1085/jgp.79.2.187 PMC Full Text View journal details
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