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PMID: 7048310 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3565-9

Cummins CM, Donahue TF, Culbertson MR

Abstract

To elucidate the molecular mechanism of frameshift suppression by the SUF2 gene of yeast, the sequences of DNA fragments carrying the SUF2-1 and suf2+ alleles of the gene and surrounding regions have been determined. Comparison of the suppressor and wild-type sequences indicates that the SUF2 gene product is a proline tRNA. Disregarding possible base modifications, we find that the wild-type suf2+ anticodon of the tRNA inferred from the DNA sequence is 3'-GGA-5'. The SUF2-1 mutation represents the insertion of a G-C base pair at a position in the gene that corresponds to the anticodon loop of the tRNA. Replacement of the wild-type suf2+ anticodon by a 3'-GGGA-5' fourbase anticodon enables the SUF2-1 tRNA to suppress the 5'-CCCU-3' four-base codons generated as the result of the his4-712 and his4-713 frameshift mutations. This nontriplet codon-anticodon interaction restores the correct reading frame and allows synthesis of a functional his4 protein.

MeSH Terms
Base Sequence Codon Genes Nucleic Acid Conformation Proline RNA, Messenger/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics Suppression, Genetic
Chemicals
Codon RNA, Messenger RNA, Transfer Proline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cummins C M
Donahue T F
Culbertson M R
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3565
Published
1982-06-00
Pages
3565-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346462
Subset
IM
Grants
NCI NIH HHS · CA26217 · United States
NIGMS NIH HHS · GM07133 · United States
Databases
GENBANK
J01365
External Links
PubMed source DOI: 10.1073/pnas.79.11.3565 PMC Full Text View journal details
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