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PMID: 7047562 Published · ppublish English Comparative Study Journal Article

Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen.

Journal of clinical microbiology ·Vol. 15 ·No. 5 ·1982-05-00 ·Pages 969-70

Minnich LL, Ray CG

Abstract

Immunofluorescence staining methods for respiratory syncytial virus antigen detection were compared. Of 50 specimens originally positive for respiratory syncytial virus by direct immunofluorescence and culture, 49 were positive by repeat direct immunofluorescence and 32 were positive by indirect immunofluorescence. Additional results obtained on specimens originally negative for respiratory syncytial virus by direct immunofluorescence, culture, or both indicate that direct immunofluorescence staining for respiratory syncytial virus antigen was more sensitive than was indirect immunofluorescence.

MeSH Terms
Antigens, Viral/analysis Epithelium/microbiology Fluorescent Antibody Technique Microbiological Techniques Nasopharynx/microbiology Respiratory Syncytial Viruses/immunology
Chemicals
Antigens, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Minnich L L
Ray C G
References (5)
5 references, click to expand
  1. Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques.
    Br Med J. 1968 Mar 9;1(5592):602-5 PMID: 4866511
  2. Rapid detection and identification of respiratory viruses by direct immunofluorescence.
    Appl Microbiol. 1970 Aug;20(2):233-9 PMID: 4098101
  3. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children.
    Infect Immun. 1974 Jul;10(1):92-101 PMID: 4367119
  4. Immunofluorescent diagnosis of acute viral infection.
    South Med J. 1975 Jun;68(6):764-6 PMID: 166450
  5. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.
    J Clin Microbiol. 1980 Sep;12(3):391-4 PMID: 6260834
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1982-05-00
Pages
969-70
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272226
Subset
IM
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