Cell structure and percent viability by a slide centrifuge technique.
作者:
Fitzgerald(M G),Hosking(C S)
状态:
发布时间1982-06-21
, 更新时间 2013-11-21
期刊:
J Clin Pathol
摘要:
It was found that a slide centrifuge (Cytospin) preparation of a cell suspension allowed a reliable assessment of not only cell structure but also the percentage of non-viable cells. The non-viable cells appeared as "smear" cells and paralleled in number the cells taking up trypan blue. Direct experiment showed the unstained viable cells in a trypan blue cell suspension remained intact in a Cytospin preparation while the cells taking up trypan blue were the "smear" cells. The non-viability of the "smear" cells was confirmed by their inability to survive in culture.