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PMID: 7040413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins.

The Journal of cell biology ·Vol. 93 ·No. 1 ·1982-04-00 ·Pages 164-74

Suprenant KA, Dentler WL

Abstract

By use of dark-field light microscopy, secretory granules isolated from the anglerfish endocrine pancreas were observed to attach to and release from microtubules assembled in vitro from brain homogenates. Secretory granules only bound to microtubules assembled in the presence of microtubule-associated proteins (MAPs) and not to microtubules assembled from purified tubulin. The addition of a MAP fraction to purified tubulin restored secretory granule binding. The secretory granules were released from MAP-containing microtubules by the addition of Mg-ATP but not by other nucleotides. The number of secretory granules bound to MAP-containing microtubules was increased in the presence of cyclic AMP. In addition to the associations of secretory granules with microtubules, MAP-containing microtubules also associated with each other. These laterally associated microtubules were dispersed by the addition of Mg-ATP. Electron micrographs confirmed that the associations between MAP-containing microtubules and secretory granules as well as the associations of microtubules with one another were mediated by the high molecular weight MAPs known to project from the surface of in-vitro-assembled microtubules.

MeSH Terms
Animals Cytoplasmic Granules/physiology,ultrastructure Fishes Islets of Langerhans/physiology,ultrastructure Microscopy, Electron Microtubule-Associated Proteins Microtubules/physiology,ultrastructure Nerve Tissue Proteins/physiology Proteins/physiology
Chemicals
Microtubule-Associated Proteins Nerve Tissue Proteins Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suprenant K A
Dentler W L
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56 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.93.1.164
Published
1982-04-00
Pages
164-74
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112120
Subset
IM
Grants
NIADDK NIH HHS · AM 00265 · United States
NIADDK NIH HHS · AM 21672 · United States
NIGMS NIH HHS · GM 20644 · United States
External Links
PubMed source DOI: 10.1083/jcb.93.1.164 PMC Full Text View journal details
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