Production and evaluation of antibody to the heat-stable enterotoxin from a human strain of enterotoxigenic Escherichia coli.

Applied and environmental microbiology ·Vol. 42 ·No. 4 ·1981-10-00 ·Pages 611-4

Kauffman PE

Abstract

Escherichia coli heat-stable enterotoxin was coupled to bovine serum albumin by a carbodiimide reagent. Antibody to the conjugate was produced by immunization of rabbits. Data from radioimmunoassay and infant mouse tests indicate the presence of antibody to the enterotoxin. The antisera can be used in a radioimmunoassay to measure enterotoxin in various fluids.

MeSH Terms

Animals Antibodies, Bacterial/analysis Antigen-Antibody Complex/analysis Binding Sites, Antibody Enterotoxins/immunology Escherichia coli/immunology Immunization Mice Radioimmunoassay Serum Albumin, Bovine/immunology

Chemicals

Antibodies, Bacterial Antigen-Antibody Complex Enterotoxins Serum Albumin, Bovine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Kauffman P E

References (8)

8 references, click to expand

Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.
J Infect Dis. 1972 Apr;125(4):407-11 PMID: 4553083
Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay.
Appl Microbiol. 1975 Jun;29(6):776-9 PMID: 1155934
Purification and chemical characterization of the heat-stable enterotoxin produced by porcine strains of enterotoxigenic Escherichia coli.
Infect Immun. 1978 Mar;19(3):1021-30 PMID: 346481
Evidence for two heat-stable enterotoxins produced by enterotoxigenic Escherichia coli.
Infect Immun. 1979 Jun;24(3):965-6 PMID: 381208
Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli.
Infect Immun. 1979 Sep;25(3):978-85 PMID: 387599
Purification and characterization of heat-stable enterotoxin produced by a strain of E. coli pathogenic for man.
J Biol Chem. 1980 May 25;255(10):4716-21 PMID: 6989817
Escherichia coli heat-stable enterotoxin: rapid method of purification and some characteristics of the toxin.
Infect Immun. 1980 May;28(2):469-74 PMID: 6995310
Physiochemical properties of a heat-stable enterotoxin produced by Escherichia coli of human origin.
Infect Immun. 1980 Jun;28(3):1051-3 PMID: 6995319

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.42.4.611-614.1981

Published

1981-10-00

Pages

611-4

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC244070

Subset

External Links

PubMed source DOI: 10.1128/aem.42.4.611-614.1981 PMC Full Text View journal details

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Production and evaluation of antibody to the heat-stable enterotoxin from a human strain of enterotoxigenic Escherichia coli.

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