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PMID: 7038689 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 3 ·1982-02-00 ·Pages 912-6

Kawakami M, Pekala PH, Lane MD, Cerami A

Abstract

Conditioned medium from cultures of mouse peritoneal exudate cells incubated wih endotoxin contains a mediator that markedly suppresses (greater than 90%) lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34) activity in differentiating 3T3-L1 mouse preadipocytes. The effect is dependent upon the amount of mediator and is evident as early as 30 min after the addition of the mediator-containing medium to 3T3-L1 cell cultures. Neither endotoxin nor conditioned medium from cultures of exudate cells not exposed to endotoxin shows the presence of the mediator. Lysates of the exudate cells are also unable to suppress the lipase activity. Increasing the amount of insulin does not reverse this suppression, even at 1000 times the concentration used for standard experiments. The lipoprotein lipase suppression mediator present in the conditioned medium of endotoxin-treated exudate cells is heat labile and has an apparent molecular weight of at least 12,000. The mediator does not inhibit lipoprotein lipase activity directly nor does it affect the half-life of enzyme activity released in the medium. The present study demonstrates that endotoxin promotes the release of a mediator from exudate cells that suppresses the activity of lipoprotein lipase in 3T3-L1 preadipocytes.

MeSH Terms
Adipose Tissue/enzymology Animals Ascitic Fluid/cytology Bacterial Toxins/pharmacology Cells, Cultured Culture Media Endotoxins/pharmacology Insulin/pharmacology Lipoprotein Lipase/antagonists & inhibitors,metabolism Macrophages/enzymology Mice Time Factors
Chemicals
Bacterial Toxins Culture Media Endotoxins Insulin Lipoprotein Lipase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kawakami M
Pekala P H
Lane M D
Cerami A
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.3.912
Published
1982-02-00
Pages
912-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345863
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.3.912 PMC Full Text View journal details
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