Home LiteratureArticle Details
PMID: 7038387 Published · ppublish English Journal Article

The target of the negative regulator of pMB1 replication overlaps with part of the repressor coding sequence.

Molecular & general genetics : MGG ·Vol. 184 ·No. 1 ·1981-00-00 ·Pages 40-5

Cesareni G

Abstract

Plasmid mutants (svir), insensitive to inhibition by the repressor of initiation of pMB1 replication, have been selected by exploiting their ability to support lambda growth in the presence of lambda repressor and inhibitor of plasmid replication. The alteration in the mechanism that controls plasmid replication causes a change in the plasmid copy number, svir mutants are dominant, as expected for mutants in the target of a repressor, but at the same time they are unable to synthesise a repressor active on the wild-type target. This lack of cross interaction between svir mutants and a co-resident wild-type plasmid results in their compatibility. These findings are explained by postulating that the target of the inhibitor of pMB1 replication coincides with part of the DNA segment that codes for the inhibitor itself. As a consequence single base pair changes in the target result in altered repressor molecules.

MeSH Terms
DNA Replication Escherichia coli/genetics Mutation Operon Phenotype Plasmids
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cesareni G
References (25)
25 references, click to expand
  1. Role of plasmid-coded RNA and ribonuclease III in plasmid DNA replication.
    Cell. 1979 Sep;18(1):61-71 PMID: 389434
  2. A minute circular DNA from Escherichia coli 15.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):992-9 PMID: 4875809
  3. Genetic analysis of the inter-relationship between plasmid replication and incompatibility.
    Mol Gen Genet. 1979 Jul 13;174(2):135-47 PMID: 386041
  4. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.
    J Bacteriol. 1975 Nov;124(2):641-9 PMID: 1102525
  5. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  6. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  7. Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.
    J Bacteriol. 1980 Jan;141(1):111-20 PMID: 6986353
  8. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.
    Methods Enzymol. 1980;65(1):363-71 PMID: 7374459
  9. Directive segregation in the basis of colE1 plasmid incompatibility.
    Nature. 1979 Oct 11;281(5731):447-52 PMID: 386132
  10. Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1421-5 PMID: 6165011
  11. A single base-pair alteration is responsible for the DNA overproduction phenotype of a plasmid copy-number mutant.
    Cell. 1981 Apr;24(1):235-42 PMID: 6263488
  12. R plasmid gene dosage effects in Escherichia coli K-12: copy mutants of the R plasmic R1drd-19.
    Plasmid. 1977 Nov;1(1):1-7 PMID: 375268
  13. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  14. Genetic and physical studies on the replication of ColE1-type plasmids.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:69-76 PMID: 383386
  15. The plasmid as a tool for plasmid genetics. I. Fine structure of the beta-lactamase gene.
    Genet Res. 1982 Dec;40(3):217-31 PMID: 6761236
  16. Incompatibility properties of Col E1 and pMB1 derivative plasmids: random replication of multicopy replicons.
    Cell. 1981 Jan;23(1):229-38 PMID: 7011568
  17. Mutational specificity of a conditional Escherichia coli mutator, mutD5.
    Mol Gen Genet. 1974;133(3):179-91 PMID: 4614067
  18. Gene regulation at the right operator (OR) of bacteriophage lambda. II. OR1, OR2, and OR3: their roles in mediating the effects of repressor and cro.
    J Mol Biol. 1980 May 15;139(2):163-94 PMID: 6447795
  19. The plasmid as a tool for plasmid genetics. II. Isolation of point mutations that affect replication of a ColE1-related plasmid.
    Genet Res. 1982 Dec;40(3):233-47 PMID: 6298062
  20. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  21. Phasmids: hybrids between ColE1 plasmids and E. coli bacteriophage lambda.
    Gene. 1982 Jan;17(1):27-44 PMID: 6281134
  22. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  23. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map.
    J Mol Biol. 1977 Jan 15;109(2):275-98 PMID: 839542
  24. Analysis of a recessive plasmid copy number mutant: evidence for negative control of Col E1 replication.
    Cell. 1979 Oct;18(2):267-75 PMID: 387257
  25. A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.
    Fed Proc. 1976 Jul;35(9):2037-43 PMID: 776705
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271192
Published
1981-00-00
Pages
40-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271192
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2