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PMID: 7034716 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proline biosynthesis in Escherichia coli. Stoichiometry and end-product identification of the reaction catalysed by glutamate semialdehyde dehydrogenase.

The Biochemical journal ·Vol. 197 ·No. 2 ·1981-08-01 ·Pages 269-74

Hayzer DJ, Leisinger T

Abstract

The stoichiometry of the oxidative phosphorylation of glutamic acid 5-semialdehyde by gamma-glutamyl phosphate reductase (glutamate semialdehyde dehydrogenase) has been established. Equimolar amounts of NADP+ and L-glutamic acid 5-semialdehyde are consumed and equimolar amounts of 5-oxiopyrroilidine-2-carboxylic acid and NADPH are formed. The end-product of the reaction is demonstrated to be 5-oxopyrrolidine-2-carboxylic acid, probably arising from the true end-product gamma-glutamyl phosphate.

MeSH Terms
Aldehyde Oxidoreductases/metabolism Chemical Phenomena Chemistry Chromatography, Thin Layer Escherichia coli/metabolism Glutamate-5-Semialdehyde Dehydrogenase NADP/metabolism Proline/biosynthesis Pyrrolidonecarboxylic Acid/metabolism
Chemicals
NADP Proline Aldehyde Oxidoreductases Glutamate-5-Semialdehyde Dehydrogenase Pyrrolidonecarboxylic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hayzer D J
Leisinger T
References (8)
8 references, click to expand
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    Biochim Biophys Acta. 1969 Dec 30;192(3):462-7 PMID: 4904678
  2. The biosynthesis of proline in Escherichia coli: phosphate-dependent glutamate -semialdehyde dehydrogenase (NADP), the second enzyme in the pathway.
    Biochim Biophys Acta. 1971 Jul 20;244(1):129-34 PMID: 4399189
  3. Improved chemical synthesis and enzymatic assay of delta-1-pyrroline-5-carboxylic acid.
    Anal Biochem. 1975 Mar;64(1):85-97 PMID: 166569
  4. Transfer of oxygen in the glutamine synthetase reaction.
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  5. Energetics of peptide formation.
    Nature. 1952 May 31;169(4309):922 PMID: 14941086
  6. Mechanism of glutamic acid activation and glutamine synthesis.
    Fed Proc. 1962 Nov-Dec;21:1013-22 PMID: 13934609
  7. ENZYMATIC SYNTHESIS OF BETA-AMINOGLUTARAMIC ACID (BETA-GLUTAMINE) BY GLUTAMINE SYNTHETASE: EVIDENCE FOR THE UTILIZATION OF BETA-AMINOGLUTARYLPHOSPHATE.
    Biochemistry. 1964 Jun;3:824-8 PMID: 14211623
  8. Properties and analysis of a stable derivative of pyrroline-5-carboxylic acid for use in metabolic studies.
    Anal Biochem. 1976 Aug;74(2):430-40 PMID: 962101
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1970269
Published
1981-08-01
Pages
269-74
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163123
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1970269 PMC Full Text View journal details
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