The proteins of the messenger RNA binding site of Escherichia coli ribosomes.

Nucleic acids research ·Vol. 9 ·No. 14 ·1981-07-24 ·Pages 3465-81

Gimautdinova OI, Karpova GG, Knorre DG, Kobetz ND

Abstract

Oligo(U) derivatives with [14C]-4-(N-2-chloroethyl-N-methylamino)benzaldehyde attached to 3'-end cis-diol group via acetal bond, p(Up)n-1UCHRCl as well as with [14C]-4-(N-2-chloroethyl-N-methylamino)benzylamine attached to 5'-phosphate via amide bond, ClRCH2NHpU(pU)6 were used to modify 70S E. coli ribosomes near mRNA binding centre. Within ternary complex with ribosome and tRNAPhe all reagents covalently bind to ribosome the extent of modification being 0.1-0.4 mole/mole 70S. p(Up)n-1UCHRCl alkylates either 30S (n=5,7) or both subunits (n=6,8). rRNA is preferentially modified within 30S subunit. ClRCH2NHpU(pU)6 alkylates both subunits the proteins being mainly modified. The distribution of the label among proteins differ for various reagents. S4, S5, S7, S9, S11, S13, S15, S18 and S21 are found to be alkylated within 30S subunit, proteins L1, L2, L6, L7/L12, L19, L31 and L32 are modified in the 50S subunit. Most proteins modified within 30S subunit are located at the "head" of this subunit and proteins modified within 50S subunit are located at the surface of the contact between this subunit and the "head" of 30S subunit at the model of Stoffler.

MeSH Terms

Benzaldehydes Benzylamines Binding Sites Escherichia coli/metabolism Kinetics Mustard Compounds Poly U/metabolism Protein Binding RNA, Messenger/metabolism RNA, Transfer/metabolism RNA, Transfer, Amino Acyl/metabolism Ribosomal Proteins/metabolism Ribosomes/metabolism

Chemicals

Benzaldehydes Benzylamines Mustard Compounds RNA, Messenger RNA, Transfer, Amino Acyl Ribosomal Proteins Poly U 4(N)-(2-chloroethyl-N-methylamino)benzylamine RNA, Transfer

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gimautdinova O I

Karpova G G

Knorre D G

Kobetz N D

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.14.3465

Published

1981-07-24

Pages

3465-81

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327364

Subset

External Links

PubMed source DOI: 10.1093/nar/9.14.3465 PMC Full Text View journal details

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The proteins of the messenger RNA binding site of Escherichia coli ribosomes.

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