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PMID: 702047 Published · ppublish English Journal Article

Leukocyte surface origin of human alpha1-acid glycoprotein (orosomucoid).

The Journal of experimental medicine ·Vol. 148 ·No. 2 ·1978-08-01 ·Pages 507-21

Gahmberg CG, Andersson LC

Abstract

Specific antibodies against human alpha1-acid glycoprotein reacted with human lymphocytes, granulocytes, and monocytes. The antigen on the leukocytes is an externally located integral membrane glycoprotein which is made by the cells and has an apparent mol wt of 52,000. It is released from cells in vitro to the culture medium. The mol wt of the soluble fragment is 41,000, which corresponds to that of alpha1-acid glycoprotein in serum and urine. Peptide mapping confirmed that the main part of the cellular membrane antigen consists of alpha1-acid glycoprotein with an additional, probably hydrophobic fragment. This finding may partially explain the increase in the serum levels of alpha1-acid glycoprotein observed in many disorders involving leukocyte proliferation. In addition, the known sequence homology of alpha1-acid glycoprotein with immunoglobulins can now be more easily understood by their origin in similar cell types.

MeSH Terms
Antigens, Surface Cyanogen Bromide Granulocytes/metabolism Humans Infectious Mononucleosis/urine Leukocytes/metabolism Lymphocytes/metabolism Membrane Proteins/blood Molecular Weight Monocytes/metabolism Orosomucoid/immunology,metabolism,urine
Chemicals
Antigens, Surface Membrane Proteins Orosomucoid Cyanogen Bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gahmberg C G
Andersson L C
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29 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.148.2.507
Published
1978-08-01
Pages
507-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2184935
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.148.2.507 PMC Full Text View journal details
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