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PMID: 7017348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.

Molecular & general genetics : MGG ·Vol. 181 ·No. 3 ·1981-00-00 ·Pages 356-62

Bendiak DS, Friesen JD

Abstract

Restriction endonuclease-generated DNA fragments of lambda polC-9 (Friesen et al. 1976) have been cloned on plasmid vehicles. Expression of genes carried by these plasmids was determined either by genetic complementation of the appropriate mutants, or in ultraviolet-irradiated cells. On the basis of these experiments we have inferred the following gene order in the four minute region of the Escherichia coli chromosome: tonA-dapD4-dapD2-rpsB-tsf-22 kilodalton protein - fir 27,000-firA-dnaE. We suggest that rpsB and tsf are in one transcriptional unit, with rpsB being promoter-proximal. We also suggest the possible position of the promoter for dnaE.

MeSH Terms
Chromosomes, Bacterial/ultrastructure Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Gene Expression Regulation Genes Genetic Linkage Operon Peptide Elongation Factors/genetics Plasmids Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Peptide Elongation Factors Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bendiak D S
Friesen J D
References (24)
24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425611
Published
1981-00-00
Pages
356-62
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425611
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