In order to identify which brain centers are involved in the control of electric organ discharge in electric fish, HRP was injected into the medullary electromotor (pacemaker) nucleus in the gymnotoid Eigenmannia. Neurons were retrogradely labeled in only a small nucleus of the mesencephalic tegmentum, herein called the prepacemaker nucleus. The prepacemaker nucleus lies just caudal and ventral to the posterior commissure and comprises at least two types of neurons.
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