Cloning of naturally occurring mixed infections of malaria parasites.

Clones have been established from a cultured isolate of Plasmodium falciparum characterized by two electrophoretic forms of glucose phosphate isomerase. Cultures initiated from diluted samples containing an estimated 1.0 or 0.5 parasite showed microscopically visible parasites after 21 days. Most of these cultures were characterized by only single enzyme forms.