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PMID: 701376 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.

The Journal of cell biology ·Vol. 79 ·No. 1 ·1978-10-00 ·Pages 281-5

Adair WS, Jurivich D, Goodenough UW

Abstract

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

MeSH Terms
Antigen-Antibody Complex Chlamydomonas/ultrastructure Electrophoresis, Polyacrylamide Gel Flagella/analysis Glycoproteins/analysis Membrane Proteins/analysis Methods Tubulin/analysis
Chemicals
Antigen-Antibody Complex Glycoproteins Membrane Proteins Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Adair W S
Jurivich D
Goodenough U W
References (9)
9 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.79.1.281
Published
1978-10-00
Pages
281-5
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110225
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.79.1.281 PMC Full Text View journal details
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