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PMID: 7011971 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Influence of complement on the chemiluminescent response of human leukocytes to immune complex.

Infection and immunity ·Vol. 29 ·No. 2 ·1980-08-00 ·Pages 335-41

Löfgren S, Tärnvik A, Carlsson J

Abstract

Vaccine bacteria of Francisella tularensis were mixed with human serum containing specific antibodies against the same organism, and the mixture was incubated at 37 degrees C for 30 min. The mixture induced a two-peak chemiluminescent response in polymorphonuclear leukocytes. The initial peak was induced by a soluble agent, formed during incubation. The formation of this agent involved the activation of complement component C5. The second peak of the chemiluminescent response was induced by the opsonized bacteria; this peak was augmented by complement component C3, whereas C5 had no influence.

MeSH Terms
Antigen-Antibody Complex Blood Complement C5/immunology Complement System Proteins/immunology Francisella tularensis/immunology Humans Immunization Immunologic Techniques Luminescent Measurements Neutrophils/immunology Opsonin Proteins/immunology
Chemicals
Antigen-Antibody Complex Complement C5 Opsonin Proteins Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Löfgren S
Tärnvik A
Carlsson J
References (24)
24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.29.2.335-341.1980
Published
1980-08-00
Pages
335-41
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC551122
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.29.2.335-341.1980 PMC Full Text View journal details
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