Rheumatoid factors in mice: plaque assay for homophile and heterophile rheumatoid factors.

Immunology ·Vol. 41 ·No. 3 ·1980-11-00 ·Pages 569-77

Dresser DW, Popham AM

Abstract

It was found that a high proportion of IgM-producing cells secrete homophile or heterophile rheumatoid factors (RF). Injection of lipopolysaccharide (LPS) into CBA mice resulted in a sharp increase in total IgM and in RF-producing cells and in some cases resulted in an increase in RF-producing cells as a proportion of total IgM. RF plaques are indirect IgM in the sense that they require development by IgG2 (anti-IgM) antibody for visualization in a standard haemolytic plaque assay. The RF response to LPS is both dose- and time-dependent.

MeSH Terms

Animals Dose-Response Relationship, Immunologic Hemolytic Plaque Technique Immunoglobulin G/immunology Immunoglobulin M/biosynthesis Lipopolysaccharides/immunology Male Mice Mice, Inbred CBA Rheumatoid Factor/biosynthesis Time Factors

Chemicals

Immunoglobulin G Immunoglobulin M Lipopolysaccharides Rheumatoid Factor

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dresser D W

Popham A M

References (19)

19 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1980-11-00

Pages

569-77

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1458158

Subset

External Links

PubMed source PMC Full Text View journal details

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Rheumatoid factors in mice: plaque assay for homophile and heterophile rheumatoid factors.

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