Home LiteratureArticle Details
PMID: 7002913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overproduction of outer membrane protein suppresses envA-induced hyperpermeability.

Journal of bacteriology ·Vol. 144 ·No. 3 ·1980-12-00 ·Pages 884-90

Grundström T, Normark S, Magnusson KE

Abstract

A quantitative study on outer membrane components was performed in a number of envelope mutants of Escherichia coli K-12 exhibition different permeability properties for antimicrobial agents. The envA1 allele causing an increased influx for both hydrophobic and hydrophilic drugs was found to be associated with a deficiency in the amount of lipopolysaccharides. The sefA1 envA1 double mutant was found to have a higher outer membrane buoyant density, apparently due to an increase in protein content. This double mutant was still low in lipopolysaccharide content.

MeSH Terms
Alleles Anti-Bacterial Agents/metabolism Bacterial Proteins/biosynthesis Cell Membrane Permeability Escherichia coli/genetics,metabolism Lipopolysaccharides/metabolism Membrane Proteins/biosynthesis Mutation Phospholipids/metabolism Suppression, Genetic
Chemicals
Anti-Bacterial Agents Bacterial Proteins Lipopolysaccharides Membrane Proteins Phospholipids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grundström T
Normark S
Magnusson K E
References (28)
28 references, click to expand
  1. Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics.
    J Bacteriol. 1969 Mar;97(3):1334-42 PMID: 4887513
  2. Mutation in Escherichia coli K-12 mediating spherelike envelopes and changes tolerance to ultraviolet irradiation and some antibiotics.
    J Bacteriol. 1969 Jun;98(3):1274-7 PMID: 4892376
  3. A new method for the extraction of R lipopolysaccharides.
    Eur J Biochem. 1969 Jun;9(2):245-9 PMID: 5804498
  4. Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents.
    Genet Res. 1970 Aug;16(1):63-78 PMID: 4922970
  5. Phenethyl alcohol as a suppressor of the envA phenotype associated with the envA gene in Escherichia coli K-12.
    J Bacteriol. 1971 Oct;108(1):51-8 PMID: 4941568
  6. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  7. Morphological mutants of Escherichia coli. Isolation and ultrastructure of a chain-forming envC mutant.
    J Gen Microbiol. 1973 Apr;75(2):409-16 PMID: 4574921
  8. The interplay of beta-lactamases and intrinsic factors in the resistance of gram-negative bacteria to penicillins and cephalosporins.
    Ann N Y Acad Sci. 1974 May 10;235(0):553-68 PMID: 4528040
  9. Characterization of lipopolysaccharides from Escherichia coli K-12 mutants.
    J Bacteriol. 1975 Feb;121(2):455-64 PMID: 1089628
  10. Outer membrane of Salmonella typhimurium: reconstitution of sucrose-permeable membrane vesicles.
    Biochem Biophys Res Commun. 1975 Jun 16;64(4):1224-30 PMID: 1095017
  11. Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants.
    J Bacteriol. 1975 Nov;124(2):942-58 PMID: 1102538
  12. Outer membrane of Salmonella typhimurium. Transmembrane diffusion of some hydrophobic substances.
    Biochim Biophys Acta. 1976 Apr 16;433(1):118-32 PMID: 769835
  13. Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels.
    J Biol Chem. 1976 Apr 10;251(7):2176-8 PMID: 773934
  14. Outer membrane of Salmonella typhimurium: accessibility of phospholipid head groups to phospholipase c and cyanogen bromide activated dextran in the external medium.
    Biochemistry. 1976 Jun 15;15(12):2561-70 PMID: 820368
  15. Septum formation-defective mutant of Escherichia coli.
    J Bacteriol. 1976 Oct;128(1):401-12 PMID: 789345
  16. Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli.
    J Bacteriol. 1977 Jan;129(1):378-87 PMID: 318645
  17. Outer membrane of Salmonella XIV. Reduced transmembrane diffusion rates in porin-deficient mutants.
    Biochem Biophys Res Commun. 1976 May 23;76(2):324-30 PMID: 800339
  18. Identification of lipopolysaccharides and phospholipids of Escherichia coli in polyacrylamide gels.
    J Bacteriol. 1977 Jul;131(1):347-55 PMID: 326766
  19. Role of a major outer membrane protein in Escherichia coli.
    J Bacteriol. 1977 Aug;131(2):631-7 PMID: 328491
  20. The tendency of smooth and rough Salmonella typhimurium bacteria and lipopolysaccharide to hydrophobic and ionic interaction, as studied in aqueous polymer two-phase systems.
    Acta Pathol Microbiol Scand B. 1977 Jun;85(3):212-8 PMID: 329639
  21. Function of the outer membrane of Escherichia coli as a permeability barrier to beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1977 Sep;12(3):368-72 PMID: 334063
  22. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3751-5 PMID: 358202
  23. Permeability of the outer membrane of bacteria.
    Angew Chem Int Ed Engl. 1979 May;18(5):337-50 PMID: 112885
  24. Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane.
    J Bacteriol. 1979 Sep;139(3):899-910 PMID: 383699
  25. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.
    J Bacteriol. 1951 Sep;62(3):293-300 PMID: 14888646
  26. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  27. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  28. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.3.884-890.1980
Published
1980-12-00
Pages
884-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294749
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.144.3.884-890.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2