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PMID: 6999016 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies specific for dengue virus type 3.

Journal of clinical microbiology ·Vol. 12 ·No. 1 ·1980-07-00 ·Pages 74-8

Dittmar D, Haines HG, Castro A

Abstract

Mouse lymphocyte hybridomas were prepared by polyethylene glycol-mediated fusion of cells from a mouse plasmacytoma line with lymphocytes from a mouse hyperimmunized with dengue virus type 3 (dengue-3). Media from 50 hybrid colonies were screened; 46 of them showed antibody activity against dengue-3-infected cells as determined by an indirect immunofluorescent antibody technique. Dengue monoclonal antibody obtained after cloning one of these colonies demonstrated activity in hemagglutination inhibition and indirect immunofluorescent antibody assays with dengue-3 antigen, but not type 1, 2, and 4 antigens. In addition, this antibody activity could be removed from culture media only by absorption with dengue-3 antigen.

MeSH Terms
Absorption Animals Antibodies, Neoplasm/biosynthesis Antibodies, Viral/biosynthesis Antibody Specificity Cell Fusion Cell Line Dengue Virus/immunology Fluorescent Antibody Technique Hemagglutination Inhibition Tests Hybrid Cells/immunology Immunoglobulin G/biosynthesis Lymphocytes/immunology Mice Plasmacytoma/immunology
Chemicals
Antibodies, Neoplasm Antibodies, Viral Immunoglobulin G
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dittmar D
Haines H G
Castro A
References (17)
17 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.12.1.74-78.1980
Published
1980-07-00
Pages
74-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC273524
Subset
IM
Grants
NIAID NIH HHS · 5R22-AI-13380-03 · United States
External Links
PubMed source DOI: 10.1128/jcm.12.1.74-78.1980 PMC Full Text View journal details
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