Home LiteratureArticle Details
PMID: 6995447 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Dimer excision in Escherichia coli in the presence of caffeine.

Journal of bacteriology ·Vol. 143 ·No. 1 ·1980-07-00 ·Pages 520-4

Rothman RH

Abstract

The observation that polA1 and recL152 mutations result in both slow pyrimidine dimer excision and large repair patch size leads to the hypothesis that patch size is directly related to the rate of excision. In this study caffeine, a known inhibitor of excision repair, was used to examine the extent of correlation between excision rate and patch size by measuring patch size in the presence of several concentrations of caffeine. Both the rate of excision and the resistance to ultraviolet radiation were reduced with increasing concentrations of caffeine after irradiation. Caffeine also inhibited the rate at which incisions were made and prolonged the time required to rejoin the discontinuities. Patch size, however, was unaffected by caffeine treatment.

MeSH Terms
Caffeine/pharmacology DNA Repair DNA, Bacterial/metabolism,radiation effects Dose-Response Relationship, Drug Escherichia coli/drug effects,metabolism Kinetics Pyrimidine Dimers/metabolism Ultraviolet Rays
Chemicals
DNA, Bacterial Pyrimidine Dimers Caffeine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rothman R H
References (20)
20 references, click to expand
  1. The effect of caffeine on the repair of ultraviolet-damaged DNA in bacteria.
    Biochim Biophys Acta. 1967;145(3):763-70 PMID: 4863908
  2. Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495-502 PMID: 4877274
  3. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  4. Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties.
    J Bacteriol. 1970 Apr;102(1):178-86 PMID: 4314477
  5. Repair deficiency in a bacterial mutant defective in DNA polymerase.
    Biochem Biophys Res Commun. 1970 Apr 8;39(1):149-55 PMID: 4908846
  6. Excision-repair properties of an Escherichia coli mutant deficient in DNA polymerase.
    Nature. 1970 May 23;226(5247):708-10 PMID: 4910149
  7. The interaction of caffeine with ultra-violet-light-irradiated DNA.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1970;17(4):395-9 PMID: 5309879
  8. Caffeine-death in Escherichia coli.
    Mol Gen Genet. 1968;102(4):316-35 PMID: 4914755
  9. Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):708-12 PMID: 5279512
  10. Ultraviolet- and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli.
    J Bacteriol. 1971 Jul;107(1):61-7 PMID: 4935330
  11. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1156-60 PMID: 4556455
  12. Heterogeneity of patch size in repair replicated DNA in Escherichia coli.
    J Mol Biol. 1972 Jun 14;67(1):1-10 PMID: 4557600
  13. Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.
    Cancer Res. 1974 Dec;34(12):3318-25 PMID: 4371955
  14. Excision-repair in mutants of Escherichia coli deficient in DNA polymerase I and/or its associated 5' leads to 3' exonuclease.
    Mol Gen Genet. 1977 Jan 7;150(1):1-12 PMID: 319338
  15. DNA polymerase I-mediated ultraviolet repair synthesis in toluene-treated Escherichia coli.
    J Biol Chem. 1978 Feb 10;253(3):665-70 PMID: 340456
  16. Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12.
    Mol Gen Genet. 1977 Oct 24;155(3):267-77 PMID: 340883
  17. Dimer excision and repair replication patch size in recL152 mutant of Escherichia coli K-12.
    J Bacteriol. 1978 Oct;136(1):444-8 PMID: 361703
  18. Pyrimidine dimer excision in Escherichia coli strains deficient in exonucleases V and VII and in the 5' leads to 3' exonuclease of DNA polymerase I.
    J Bacteriol. 1979 Jan;137(1):234-42 PMID: 368015
  19. Inhibition of deoxyribonucleic acid repair in Escherichia coli by caffeine and acriflavine after ultraviolet irradiation.
    J Bacteriol. 1979 Aug;139(2):671-4 PMID: 378983
  20. Deoxyribonucleic acid repair in Escherichia coli mutants deficient in the 5'----3' exonuclease activity of deoxyribonucleic acid polymerase I and exonuclease VII.
    J Bacteriol. 1977 May;130(2):667-75 PMID: 400786
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.1.520-524.1980
Published
1980-07-00
Pages
520-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294281
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.143.1.520-524.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2