Isolation and properties of Salmonella typhimurium mutants defective in enolase.

Mutants defective in enolase have been isolated and characterized in Salmonella typhimurium LT-2, and their properties of growth have been studied in different carbon sources. They do not grow in a mixture of phosphoenolpyruvate and 3-phosphoglycerate, thereby not furnishing any information as to how isolated mutants defective in phosphoglyceromutase. The difference between the concentrations of 3-phosphoglycerate and 2-phosphoglycerate inside the cells of the mutants when they are exposed to glucose or glycerol suggests that the 3PGA in equilibrium 2PGA reaction catalysed by phosphoglycertomutase in vivo is not in equilibrium. The transduction experiments for the genetic mapping of the gene affected show that eno becomes by minute 92 the chromosome of S. typhimurium.