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PMID: 6989798 Published · ppublish English Journal Article

Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12.

Journal of bacteriology ·Vol. 142 ·No. 1 ·1980-04-00 ·Pages 120-30

Roehl RA, Vinopal RT

Abstract

Most strains of Escherichia coli K-12 are unable to use the enzyme IIA/IIB (enzyme IIMan) complex of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in anaerobic growth and therefore cannot utilize glucosamine anaerobically. Introduction into these strains of a ptsG mutation, which eliminates activity of the enzyme IIIGlc/IIB' complex of the PTS, resulted in inability to grow anaerobically on glucose and mannose. Derivative strains able to grow anaerobically on glucosamine had mutations at a locus close to man, the gene coding for phosphomannose isomerase, and had higher enzyme IIA/IIB activities during anaerobic growth than did the parental strain. These results establish a locus affecting function of enzyme IIA/IIB that maps distant from ptsM, the probable structural gene for enzyme IIB.

MeSH Terms
Anaerobiosis Biological Transport Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology,genetics Genes Glucosamine/metabolism Glucose/metabolism Mannose/metabolism Mutation Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism
Chemicals
Phosphoenolpyruvate Sugar Phosphotransferase System Glucose Glucosamine Mannose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roehl R A
Vinopal R T
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.142.1.120-130.1980
Published
1980-04-00
Pages
120-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293914
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.142.1.120-130.1980 PMC Full Text View journal details
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