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PMID: 6987669 Published · ppublish English Journal Article

Inhibition of RNA polymerase activity by the Escherichia coli protein biosynthesis elongation factor Ts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 866-9

Biebricher CK, Druminski M

Abstract

The transcribing activity of DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) from Escherichia coli is inhibited in vitro by addition of preparations of elongation factor Ts purified to homogeneity. The inhibitory activity of elongation factor Ts on the RNA polymerase activity and the enzymatic activity of elongation factor Ts show the same temperature sensitivity. The extent of inhibition is strongly dependent on the template used for transcription. A mechanism for the control of RNA synthesis in vivo based on this inhibition found in vitro is proposed.

MeSH Terms
Chemical Precipitation DNA-Directed RNA Polymerases/antagonists & inhibitors Escherichia coli/enzymology,metabolism Guanosine Pentaphosphate/pharmacology Peptide Elongation Factors/pharmacology Protein Binding
Chemicals
Peptide Elongation Factors Guanosine Pentaphosphate DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biebricher C K
Druminski M
References (20)
20 references, click to expand
  1. Studies on the polypeptide elongation factors from E. coli. IV. Crystalline Tu-GTP, Tu-Gpp(CH2)p, and phenylalanyl-tRNA-Tu-GTP complex.
    J Biochem. 1974 Aug;76(2):283-92 PMID: 4609970
  2. Studies on the polypeptide elongation factors from E. coli. V. Properties of various complexes containing EF-Tu and EF-Ts.
    J Biochem. 1974 Aug;76(2):293-306 PMID: 4609971
  3. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  4. Content of elongation factor Tu in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4780-4 PMID: 1108000
  5. The mechanism of action of ppGpp on rRNA synthesis in vitro.
    Cell. 1976 May;8(1):123-8 PMID: 8211
  6. Multiple modes of ribosomal RNA transcription in vitro.
    Cell. 1976 Aug;8(4):605-9 PMID: 782727
  7. [Protein chromatography on calcium phosphate. I. Purification of nitrate reductase from wheat leaves].
    Biochem Z. 1958;330(2):141-59 PMID: 13546188
  8. A new method for the purification of RNA-polymerase.
    Biochem Biophys Res Commun. 1967 Mar 21;26(6):639-44 PMID: 6030974
  9. The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.
    J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
  10. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
  11. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.
    J Biol Chem. 1970 May 10;245(9):2309-18 PMID: 4315151
  12. Immunochemical distinction between the Escherichia coli polypeptide chain elongation factors Tu and Ts.
    Biochemistry. 1970 Oct 13;9(21):4233-6 PMID: 4989846
  13. Factor necessary for ribosomal RNA synthesis.
    Nature. 1970 Nov 21;228(5273):748-51 PMID: 4919595
  14. Studies on the purification and properties of factor Tu from E. coli.
    Arch Biochem Biophys. 1970 Nov;141(1):26-37 PMID: 4921066
  15. Covalent attachment of nucleic acids to agarose for affinity chromatography.
    Biochemistry. 1971 Feb 2;10(3):424-7 PMID: 5543969
  16. Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.
    Eur J Biochem. 1972 Apr 24;26(4):474-81 PMID: 5063565
  17. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7 PMID: 4624757
  18. Studies on polypeptide elongation factors from Escherichia coli. II. Purification of factors Tu-guanosine diphosphate, Ts, and Tu-Ts, and crystallization of Tu-guanosine diphosphate and Tu-Ts.
    J Biol Chem. 1972 Nov 10;247(21):7029-37 PMID: 4563073
  19. Control of ribosomal RNA synthesis in vitro.
    Nature. 1973 Jul 6;244(5410):15-8 PMID: 4582479
  20. Macromolecular composition during steady-state growth of Escherichia coli B-r.
    J Bacteriol. 1974 Jul;119(1):270-81 PMID: 4600702
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.866
Published
1980-02-00
Pages
866-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348382
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.866 PMC Full Text View journal details
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