Generation of F1 hybrid cytotoxic T lymphocytes specific for self H-2.

Cytotoxic T lymphocytes specific for self H-2 antigens are generated by murine F1 hybrid (H-2 heterozygous) spleen cells cultured with irradiated parental (H-2 homozygous) splenocytes. The effectors bind to heterozygous and homozygous cells bearing the appropriate H-2 alleles but only lyse homozygous targets. Autoreactivity for membrane-bound molecules of normal cells may be a mechanism for regulating cellular interactions.