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PMID: 6968912 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5419-23

Krolick KA, Villemez C, Isakson P, Uhr JW, Vitetta ES

Abstract

Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface immunoglobulins on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the two allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. The results demonstrate that A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.

MeSH Terms

Animals Antibodies, Anti-Idiotypic Antibody Specificity Antilymphocyte Serum B-Lymphocytes/drug effects,immunology Clone Cells/immunology Hybrid Cells/immunology Immunoglobulin Allotypes/immunology Immunoglobulin D/immunology Immunoglobulin Idiotypes/immunology Immunoglobulin M/immunology Mice Neoplasms, Experimental/immunology Peptide Fragments/pharmacology Protein Biosynthesis Ricin/administration & dosage,pharmacology

Chemicals

Antibodies, Anti-Idiotypic Antilymphocyte Serum Immunoglobulin Allotypes Immunoglobulin D Immunoglobulin Idiotypes Immunoglobulin M Peptide Fragments Ricin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Krolick K A

Villemez C

Isakson P

Uhr J W

Vitetta E S

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.9.5419

Published

1980-09-00

Pages

5419-23

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350070

Subset

Grants

NIAID NIH HHS · AI-11851 · United States

NIAID NIH HHS · AI-12789 · United States

NCI NIH HHS · CA-23115 · United States

External Links

PubMed source DOI: 10.1073/pnas.77.9.5419 PMC Full Text View journal details

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Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.

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