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PMID: 6957876 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neuropeptide Y: complete amino acid sequence of the brain peptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 18 ·1982-09-00 ·Pages 5485-9

Tatemoto K

Abstract

The amino acid sequence of neuropeptide Y, a 36-residue peptide recently isolated from porcine brain, has been determined by using high performance liquid chromatography for separation of its tryptic and chymotryptic fragments and subsequent sequence analysis of the isolated fragments by an improved dansyl Edman subtractive technique. The amino acid sequence of neuropeptide Y has been found to be: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala-Arg-Tyr -Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2. Neuropeptide Y has a high degree of sequence homology with peptide YY (70%), the newly isolated porcine intestinal peptide, and pancreatic polypeptide (50%). It is therefore proposed that neuropeptide Y, peptide YY, and pancreatic polypeptide are members of a newly recognized peptide family.

MeSH Terms

Amino Acid Sequence Animals Brain Chemistry Chymotrypsin Dansyl Compounds Nerve Tissue Proteins Neuropeptide Y Peptide Fragments/analysis Swine Trypsin

Chemicals

Dansyl Compounds Nerve Tissue Proteins Neuropeptide Y Peptide Fragments Chymotrypsin Trypsin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Tatemoto K

References (24)

24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.18.5485

Published

1982-09-00

Pages

5485-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC346928

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.18.5485 PMC Full Text View journal details

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