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PMID: 6950411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 12 ·1981-12-00 ·Pages 7727-31

Young BD, Ferguson-Smith MA, Sillar R, Boyd E

Abstract

A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of variation in the range 1-2%. At this level of resolution the contribution of many of the human chromosomes can be evaluated separately. Significant and reproducible differences between normal individuals have been detected and have been correlated with differences in the centric heterochromatin of certain chromosomes as revealed in their C-banded karyotypes.

MeSH Terms

Cell Separation/methods Cells, Cultured Chromosome Banding/methods Chromosomes, Human/ultrastructure Flow Cytometry/methods Humans Karyotyping/methods Lymphocytes/ultrastructure

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Young B D

Ferguson-Smith M A

Sillar R

Boyd E

References (8)

8 references, click to expand

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Proc Natl Acad Sci U S A. 1975 Apr;72(4):1231-4 PMID: 1055399
Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region.
Am J Hum Genet. 1977 Sep;29(5):490-502 PMID: 70995
Slit-scan flow cytometry of mammalian chromosomes.
J Histochem Cytochem. 1979 Jan;27(1):441-4 PMID: 374608
Fluorescence polarization and pulse width analysis of chromosomes by a flow system.
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Measurement and purification of human chromosomes by flow cytometry and sorting.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1382-4 PMID: 286320
A new method for the preparation of metaphase chromosomes for flow analysis.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.12.7727

Published

1981-12-00

Pages

7727-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349343

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.12.7727 PMC Full Text View journal details

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