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PMID: 6946459 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Homologous species restriction in lysis of erythrocytes by terminal complement proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 5118-21

Hänsch GM, Hammer CH, Vanguri P, Shin ML

Abstract

The cytolytic efficiency of the terminal complement protein complex, C5b-9, varies with the species of origin of C8 and C9. In the present study, we explored the susceptibility of erythrocytes from various species to lysis by C5b6,7 plus C8 and C9 from different species. EC5b6,7 intermediates were prepared on human, guinea pig, rabbit, mouse, and rat erythrocytes with human C5b6 and guinea pig C7. The degree of lysis of these intermediates by C8 and C9 was found to vary widely depending on the species of the proteins and the target cells. In all cases, lysis was least efficient when C8 and C9 were homologous with respect to the target cell species. This effect was mostly attributable to C9. The inefficient lysis in a homologous system is not due to a failure of C9 binding. Rather, the poor lysis in the homologous system may be attributable to inefficient insertion or channel formation.

MeSH Terms
Animals Complement Activation Complement C5/physiology Complement C6/physiology Complement C8/physiology Complement C9/physiology Complement System Proteins/physiology Erythrocytes/immunology Guinea Pigs Hemolysis Humans Mice Rabbits Rats Species Specificity
Chemicals
Complement C5 Complement C6 Complement C8 Complement C9 Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hänsch G M
Hammer C H
Vanguri P
Shin M L
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.5118
Published
1981-08-00
Pages
5118-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320344
Subset
IM
Grants
NINDS NIH HHS · 1 R01 NS 15662-01 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.8.5118 PMC Full Text View journal details
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