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PMID: 6945572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription and processing of adenoviral RNA by extracts from HeLa cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4092-6

Weingärtner B, Keller W

Abstract

Concentrated extracts from HeLa cells prepared by the procedure of Sugden and Keller [Sugden, B. & Keller, W. (1973) J. Biol. Chem. 248, 3777-3788] can synthesize RNA molecules of discrete lengths in the presence of DNA templates carrying complete viral transcription units. Accurate initiation of transcription was shown to occur at the "major late" promoter and at the promoter for the "early region III" transcription unit of the adenovirus 2 genome. A cloned fragment of adenovirus 2 DNA containing the early region III transcription unit directs the synthesis of discrete RNA species, many of which correspond in length to spliced early region III mRNAs isolated from adenovirus 2-infected HeLa cells. As shown by hybridization/nuclease S2 analysis, RNA splicing and the formation of specific 3' ends are also taking place in vitro.

MeSH Terms
Adenoviruses, Human/genetics Cell-Free System HeLa Cells Humans Molecular Weight RNA Splicing RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weingärtner B
Keller W
References (38)
38 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  3. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  4. Amount of viral DNA in the genome of cells transformed by adenovirus type 2.
    J Mol Biol. 1973 Jan;73(1):125-30 PMID: 4734785
  5. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  6. Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.
    J Biol Chem. 1973 Jun 10;248(11):3777-88 PMID: 4708091
  7. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  10. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  11. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
    Cell. 1977 Sep;12(1):1-8 PMID: 902310
  12. Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions.
    Cell. 1977 Sep;12(1):9-21 PMID: 902321
  13. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
  14. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  15. Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 PMID: 276858
  16. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  17. In vitro RNA-RNA splicing in adenovirus 2 mRNA formation.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5344-8 PMID: 281684
  18. Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing.
    Cell. 1978 Dec;15(4):1477-93 PMID: 729004
  19. RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):160-4 PMID: 284328
  20. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
    J Biol Chem. 1979 Jul 10;254(13):6163-73 PMID: 447704
  21. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  22. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  23. Synthesis and processing of adenoviral RNA in isolated nuclei.
    J Virol. 1979 Nov;32(2):394-403 PMID: 501800
  24. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  25. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  26. Transcription units for mRNA production in eukaryotic cells and their DNA viruses.
    Prog Nucleic Acid Res Mol Biol. 1979;22:327-53 PMID: 523634
  27. RNA synthesis in isolated nuclei: identification and comparison of adenovirus 2 encoded transcripts synthesized in vitro and vivo.
    J Mol Biol. 1979 Nov 25;135(1):171-97 PMID: 529286
  28. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  29. Transcription maps of adenovirus.
    Methods Enzymol. 1980;65(1):750-68 PMID: 6246372
  30. A mechanism for RNA splicing.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1877-9 PMID: 6246511
  31. Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2424-8 PMID: 6930642
  32. In vitro processing of intervening sequences in the precursors of messenger ribonucleic acid for adenovirus 2 deoxyribonucleic acid binding protein.
    Biochemistry. 1980 Jun 10;19(12):2719-23 PMID: 6249339
  33. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  34. Biogenesis, structures, and sites of encoding of the 5' termini of adenovirus-2 mRNAs.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:415-28 PMID: 6933038
  35. Nucleotide sequence of the EcoRI D fragment of adenovirus 2 genome.
    Nucleic Acids Res. 1980 May 24;8(10):2173-92 PMID: 6253880
  36. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  37. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
    J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
  38. Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
    J Mol Biol. 1980 Dec 15;144(3):377-86 PMID: 7253021
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.7.4092
Published
1981-07-00
Pages
4092-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319732
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.7.4092 PMC Full Text View journal details
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