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PMID: 6943574 Published · ppublish English Comparative Study Journal Article

Comparison of poly(A)-mRNA prepared from membranes and free polyribosomes of mouse liver.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3697-701

Clissold PM, Mason PJ, Bishop JO

Abstract

We have compared poly(A)-mRNA isolated from mouse liver endoplasmic reticulum (ER) membranes (ER mRNA) with that from free polyribosomes (free mRNA) by translation in a cell-free system and by mRNA--cDNA reassociation analysis. The partitioning of certain translation products between the two fractions suggests that the physical contamination of the free mRNA by the ER is 10%, and contamination of the ER by the free is 1%. Reassociation crossreactions suggest that no sequences are totally absent from either fraction. In particular, the rare sequences are shared between the two fractions. Among the more abundant sequences, we detect three groups: (i) a group of about 10 sequences that are relatively abundant in both fractions; (ii) a group that occurs mostly in the free fraction; (iii) a group of about 12 sequences, 6 or 7 of them encoding secretory polypeptides. The members of this group are largely or entirely confined to the ER fraction. Secretory proteins are almost entirely absent from the products of th free mRNA fraction. Quantitation of individual mRNA sequences by hybridization with cDNA clones shows that a range of sequence concentrations exists within the abundant class of ER mRNA.

MeSH Terms
Animals Cell-Free System Cytosol/analysis Endoplasmic Reticulum/analysis Mice Microsomes, Liver/analysis Nucleic Acid Hybridization Poly A/genetics Polyribosomes/analysis Protein Biosynthesis Proteins/genetics RNA, Messenger/genetics
Chemicals
Proteins RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clissold P M
Mason P J
Bishop J O
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.6.3697
Published
1981-06-00
Pages
3697-701
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319638
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.6.3697 PMC Full Text View journal details
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