Gravitational field enhances permeability of biological membranes to sucrose: an experimental refutation of sucrose-space hypothesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3441-5

Sitaramam V, Sarma MK

Abstract

Isotonic conditions for the integrity of subcellular organelles are shown to be remarkably influenced by the concentration of sucrose present during their isolation by centrifugation. Using the technique of enzyme osmometry, we show that the content of sucrose in synaptosomes reflects nearly total equilibration across the membrane during centrifugation, due to altered permeability of membranes. Presence of sucrose in the matrix space of mitochondria, as demonstrated by enzyme osmometry of matrix enzymes, indicates that the sucrose-space hypothesis is invalid.

MeSH Terms

Animals Cell Compartmentation Centrifugation Cerebral Cortex/enzymology Gravitation Intracellular Membranes/physiology L-Lactate Dehydrogenase/metabolism Mitochondria/enzymology Osmosis Permeability Rats Sucrose Synaptosomes/enzymology Temperature

Chemicals

Sucrose L-Lactate Dehydrogenase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sitaramam V

Sarma M K

References (25)

25 references, click to expand

Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Lipid domains in membranes. Evidence derived from structural perturbations induced by free fatty acids and lifetime heterogeneity analysis.
J Biol Chem. 1980 Feb 25;255(4):1286-95 PMID: 7354027
Colorimetric estimation of ketopentoses and ketohexoses.
Biochem J. 1956 Aug;63(4):542-8 PMID: 13355847
Some permeability properties of isolated rat liver cell mitochondria.
J Gen Physiol. 1956 Sep 20;40(1):47-71 PMID: 13357737
The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium.
Biochem J. 1957 May;66(1):79-91 PMID: 13426112
Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase.
J Neurochem. 1962 Jan-Feb;9:23-35 PMID: 13884490
Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation.
Physiol Rev. 1962 Jul;42:467-517 PMID: 14463788
Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.
Biochim Biophys Acta. 1950 Jan;4(1-3):211-4 PMID: 15403927
The osmotic behavior and permeability to non-electrolytes of mitochondria.
Arch Biochem Biophys. 1955 Sep;58(1):52-67 PMID: 13259676
The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').
Biochem J. 1964 Feb;90(2):293-303 PMID: 5834239
Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients.
Biochem J. 1964 Jul;92(1):184-205 PMID: 4378797
The osmotically sensitive potassium and sodium compartments of synaptosomes.
Biochem J. 1967 Jul;104(1):148-57 PMID: 6035507
On the mechanism of non-electrolyte permeation through lipid bilayers and through biomembranes.
Biochim Biophys Acta. 1971 Jun 1;233(3):610-8 PMID: 5165435
The fluid mosaic model of the structure of cell membranes.
Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
Osmotic pressure and ATPase activity of rat liver mitochondria.
Arch Biochem Biophys. 1972 Sep;152(1):78-82 PMID: 4262874
The opposing effects of pressure and general anaesthetics on the cation permeability of liposomes of varying lipid composition.
Biochim Biophys Acta. 1973 Apr 25;307(1):42-57 PMID: 4736379
Studies on the transport of glucose from disaccharides by hamster small intestine in vitro. I. Evidence for a disaccharidase-related transport system.
Biochim Biophys Acta. 1973 May 25;307(3):613-26 PMID: 4718809
Behaviour of rat-liver mitochondria during centrifugation in a sucrose gradient.
Mol Cell Biochem. 1974 Aug 1;4(1):21-9 PMID: 4371769
Mechanisms of H2O2 formation by leukocytes. Evidence for a plasma membrane location.
Arch Biochem Biophys. 1975 Aug;169(2):428-35 PMID: 1180558
Stabilizing effect of sucrose on leakage of cytoplasm from rat brain synaptosomes in saline media.
J Neurochem. 1977 Jun;28(6):1403-5 PMID: 874506
The permeability properties of the lysosomal membrane.
Biochim Biophys Acta. 1977 Nov 14;472(3-4):419-49 PMID: 20954
Protein synthesis by synaptosomes from rat brain: the effect of centrifugal forces.
Exp Brain Res. 1978 Nov 15;33(3-4):325-35 PMID: 729658
Effect of temperature and ionophores on the permeability of synaptosomes.
J Neurochem. 1979 Jul;33(1):309-17 PMID: 458457
Loss of cell constituents from hepatocytes on centrifugation.
Biochem J. 1979 Jun 15;180(3):685-8 PMID: 486144
Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particles.
Biochem J. 1955 Mar;59(3):426-33 PMID: 14363112

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.6.3441

Published

1981-06-00

Pages

3441-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319584

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.6.3441 PMC Full Text View journal details

Analysis Services

Gravitational field enhances permeability of biological membranes to sucrose: an experimental refutation of sucrose-space hypothesis.

Contact

WeChat Official Account