Home LiteratureArticle Details
PMID: 6941299 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Micropinocytic ingestion of glycosylated albumin by isolated microvessels: possible role in pathogenesis of diabetic microangiopathy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2393-7

Williams SK, Devenny JJ, Bitensky MW

Abstract

Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of albumin and glycosylated albumin with endothelium was studied with a double-label fluorescence assay of micropinocytosis. When glycosylated albumin was present at a concentration of 6% with respect to total albumin (the level found in "non diabetic" serum), only glycosylated albumin was ingested. At higher concentrations of glycosylated albumin (those found in diabetic serum), both albumin and glycosylated albumin are ingested. Glycosylation of endothelial membrane components results in stimulated ingestion of glycosylated albumin, persistent exclusion of unmodified albumin, and unaltered micropinocytic ingestion of native ferritin. These results indicate that nonenzymatic glycosylation of serum albumin may result in rapid vesicle-mediated extravasation of albumin. Chronic microvascular leakage of glycosylated albumin could contribute to the pathogenesis of diabetic microangiopathy.

MeSH Terms
Animals Diabetes Mellitus/metabolism Diabetic Angiopathies/etiology Endothelium/metabolism Glycoproteins/metabolism Membrane Proteins/metabolism Microcirculation/metabolism Pinocytosis Rats Serum Albumin/metabolism Structure-Activity Relationship
Chemicals
Glycoproteins Membrane Proteins Serum Albumin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams S K
Devenny J J
Bitensky M W
References (35)
35 references, click to expand
  1. Diabetic retinopathy studied by fluorescein angiography.
    Ophthalmologica. 1965;150(1):5-17 PMID: 5863397
  2. Exclusion chromatography.
    Arch Biochem Biophys. 1962 Sep;Suppl 1:157-68 PMID: 13942331
  3. Studies of an unusual hemoglobin in patients with diabetes mellitus.
    Biochem Biophys Res Commun. 1969 Aug 22;36(5):838-43 PMID: 5808299
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Hemoglobin components in patients with diabetes mellitus.
    N Engl J Med. 1971 Feb 18;284(7):353-7 PMID: 5539916
  6. Characterization and analysis of frog photoreceptor membranes.
    J Gen Physiol. 1971 Sep;58(3):225-37 PMID: 4255372
  7. The actions of hormones on the adenyl cyclase system.
    Adv Biochem Psychopharmacol. 1970;3:185-208 PMID: 4399634
  8. The determination of lipoprotein lipase activity: effect of different albumin preparations.
    Clin Biochem. 1972 Jun;5(2):109-14 PMID: 5039592
  9. Fluorescent labeling of proteins. A new methodology.
    Biochem Biophys Res Commun. 1973 Oct 1;54(3):899-906 PMID: 4148130
  10. On the specificity of antibodies.
    Science. 1975 Jan 17;187(4172):130-7 PMID: 46122
  11. The isolation and culture of capillary endothelium from epididymal fat.
    Microvasc Res. 1975 Nov;10(3):286-97 PMID: 175243
  12. Stability studies on fluorescein isothiocyanate--bovine serum albumin conjugate.
    Anal Biochem. 1976 Jun;73(2):280-9 PMID: 962043
  13. Nonenzymatic glycosylation of hemoglobin.
    J Biol Chem. 1977 May 10;252(9):2998-3002 PMID: 856810
  14. Relationship of microvascular disease in diabetes to metabolic control.
    Diabetes. 1977 Aug;26(8):760-9 PMID: 885298
  15. A fluorescense assay for micropinocytosis in isolated capillary endothelium.
    Microvasc Res. 1977 Jul;14(1):67-80 PMID: 895547
  16. Blood glucose control and glomerular capillary basement membrane thickening in experimental diabetes.
    Br Med J. 1977 Sep 3;2(6087):605-7 PMID: 901998
  17. The microcirculatory society Eugene M. Landis award lecture. Transport pathways through capillary endothelium.
    Microvasc Res. 1978 Jan;15(1):123-35 PMID: 634152
  18. Relation of diabetic control to development of microvascular complications.
    Diabetologia. 1978 Sep;15(3):143-52 PMID: 359393
  19. Nonenzymatically glucosylated albumin. In vitro preparation and isolation from normal human serum.
    J Biol Chem. 1979 Feb 10;254(3):595-7 PMID: 762083
  20. Movement of interstitially microinjected 125I-labeled albumin into blood capillaries of rat skeletal muscle demonstrated with electron microscopic autoradiography.
    Microvasc Res. 1978 Nov;16(3):354-61 PMID: 748718
  21. Transport of steroid hormones through the rat blood-brain barrier. Primary role of albumin-bound hormone.
    J Clin Invest. 1979 Jul;64(1):145-54 PMID: 447850
  22. Carrier-mediated transport of thyroid hormones through the rat blood-brain barrier: primary role of albumin-bound hormone.
    Endocrinology. 1979 Sep;105(3):605-12 PMID: 467322
  23. Evidence of active transport (filtration?) of plasma proteins across the capillary walls in muscle and subcutis.
    Acta Physiol Scand Suppl. 1979;463:105-10 PMID: 289281
  24. Nonenzymatic glucosylation of serum proteins in diabetes mellitus.
    Diabetes. 1979 Nov;28(11):1011-4 PMID: 488539
  25. Metabolic studies on the micropinocytic process in endothelial cells.
    Microvasc Res. 1979 Sep;18(2):175-84 PMID: 491985
  26. Nonenzymatic glycosylation of erythrocyte membrane proteins. Relevance to diabetes.
    J Clin Invest. 1980 Apr;65(4):896-901 PMID: 7358849
  27. Exclusion of albumin from vesicular ingestion by isolated microvessels.
    Microvasc Res. 1980 Jan;19(1):127-30 PMID: 7360045
  28. Increased glycosylation of serum albumin in diabetes mellitus.
    Diabetes. 1980 Jun;29(6):417-22 PMID: 6991333
  29. Nonenzymatic glycosylation of proteins. A warning.
    J Biol Chem. 1980 Jul 25;255(14):6717-20 PMID: 6156163
  30. A fiber matrix model of capillary permeability.
    Microvasc Res. 1980 Jul;20(1):96-9 PMID: 7412590
  31. Quantitative determination of deoxyribonucleic acid from cells collected on filters.
    Anal Biochem. 1980 Sep 1;107(1):17-20 PMID: 6449161
  32. Disruption of blood-retinal barrier in experimental diabetic rats: an electron microscopic study.
    Exp Eye Res. 1980 Apr;30(4):401-10 PMID: 7449869
  33. Isolation and characterization of brain endothelial cells: morphology and enzyme activity.
    J Neurochem. 1980 Aug;35(2):374-81 PMID: 6108994
  34. Plaque formation and isolation of pure lines with poliomyelitis viruses.
    J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
  35. The catalytic and regulatory properties of enzymes.
    Annu Rev Biochem. 1968;37:359-410 PMID: 4877056
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2393
Published
1981-04-00
Pages
2393-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319352
Subset
IM
Grants
NIADDK NIH HHS · R01 AM-26412 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2393 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2