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PMID: 6941281 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Site of attachment of retinal in bacteriorhodopsin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2225-9

Bayley H, Huang KS, Radhakrishnan R, Ross AH, Takagaki Y, Khorana HG

Abstract

The identity of the lysine residue in bacteriorhodopsin to which the chromophore, retinal, is attached as a Schiff's base has been reinvestigated. Retinal is now shown to be linked to lysine-216 and not lysine-41, as had been concluded previously. The retinal in purple membrane was replaced by [15-3H]retinal, the Schiff's base linkage was reduced with NaBH4 while the sample was illuminated, and the resulting [retinyl-3H]bacterio-opsin was cleaved with CNBr. The radioactivity was present exclusively in the COOH-terminal peptide (amino acids 210--248). Sequence analysis showed that the [3H]retinal was attached to lysine-216. The same site was labeled when purple membrane was reduced with NaBH4 in the light at pH greater than 10 or at pH 8 and when membranes modified with ether or solubilized with hexadecyltrimethylammonium bromide were reduced in the dark. Our finding places of Schiff's base linkage close to the midpoint of the putative membrane-spanning alpha-helix that is directly connected to the COOH terminus of bacteriorhodopsin.

MeSH Terms
Amino Acid Sequence Bacteriorhodopsins Carotenoids Halobacterium Oxidation-Reduction Peptide Fragments Protein Binding Protein Conformation Retinaldehyde Vitamin A/analogs & derivatives
Chemicals
Peptide Fragments Vitamin A Carotenoids Bacteriorhodopsins Retinaldehyde
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bayley H
Huang K S
Radhakrishnan R
Ross A H
Takagaki Y
Khorana H G
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2225
Published
1981-04-00
Pages
2225-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319317
Subset
IM
Grants
NIAID NIH HHS · AI11479 · United States
NCI NIH HHS · T32 CA09112 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2225 PMC Full Text View journal details
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