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PMID: 6940190 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular weight of the membrane C5b-9 complex of human complement: characterization of the terminal complex as a C5b-9 monomer.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1818-22

Bhakdi S, Tranum-Jensen J

Abstract

The hydrodynamic properties of the detergent-solubilized, terminal membrane complex of serum complement components C5-C9 [C5b-9(m)] were studied to obtain an estimate of its molecular weight. In a solution of Triton X-100/deoxycholate, the protein complex binds 17% Triton X-100 and 11% deoxycholate by weight. The sedimentation coefficient of the protein-detergent complex is 26 S as determined by sucrose density gradient ultracentrifugation, and gel filtration indicated a molecular radius of 11 nm. It was ascertained by electron microscopy that these hydrodynamic parameters apply to mono-dispersed C5b-9(m) complexes, which were observed as nonaggregated, hollow protein cylinders and were identical to the complement "lesions" formed on target membranes. The calculated molecular weight of the protein-detergent complex is approximately 1,286,300 to which the protein moiety contributes approximately 1,000,000. The results indicate that the C5b-9(m) complex formed on biological membranes is a monomer entity of the C5-C9 complement components.

MeSH Terms
Complement Membrane Attack Complex Complement System Proteins Deoxycholic Acid Detergents Humans Macromolecular Substances Membranes/immunology Microscopy, Electron Molecular Weight Octoxynol Polyethylene Glycols Protein Binding Protein Conformation
Chemicals
Complement Membrane Attack Complex Detergents Macromolecular Substances Deoxycholic Acid Polyethylene Glycols Octoxynol Complement System Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhakdi S
Tranum-Jensen J
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1818
Published
1981-03-00
Pages
1818-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319226
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.3.1818 PMC Full Text View journal details
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