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PMID: 6935672 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Somatic cell genetic analysis of regulation of expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6621-3

Sinensky M, Armagast S, Mueller G, Torget R

Abstract

Three new types of regulatory somatic cell mutants defective in expression of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase are described. They include a recessive mutant with abnormally high enzyme activity, apparently defective in degradation of the enzyme, and one, phenotypically a mevalonate auxotroph, that maintains permanently repressed levels of enzyme activity and are recessive, constitutive mutants. These mutants offer means for analyses of the mechanism of regulation of expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase in cultured fibroblasts.

MeSH Terms
Animals Cells, Cultured Cholesterol/pharmacology Cricetinae Drug Resistance Female Gene Expression Regulation/drug effects Hydroxycholesterols/pharmacology Hydroxymethylglutaryl CoA Reductases/genetics Mevalonic Acid/metabolism Ovary
Chemicals
Hydroxycholesterols Cholesterol Hydroxymethylglutaryl CoA Reductases Mevalonic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sinensky M
Armagast S
Mueller G
Torget R
References (13)
13 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6621
Published
1980-11-00
Pages
6621-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350338
Subset
IM
Grants
NCI NIH HHS · CA20810 · United States
NIGMS NIH HHS · GM24732 · United States
NICHD NIH HHS · HD-02080 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.11.6621 PMC Full Text View journal details
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