A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6368-70

Hjelmeland LM

Abstract

The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.

MeSH Terms

Animals Cholic Acids/chemical synthesis,pharmacology Detergents/chemical synthesis,pharmacology Membrane Proteins/isolation & purification Mice Microsomes, Liver/analysis Protein Denaturation/drug effects Spectrum Analysis Surface-Active Agents/chemical synthesis

Chemicals

Cholic Acids Detergents Membrane Proteins Surface-Active Agents 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Hjelmeland L M

References (9)

9 references, click to expand

The fluid mosaic model of the structure of cell membranes.
Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
Solubilization of membranes by detergents.
Biochim Biophys Acta. 1975 Mar 25;415(1):29-79 PMID: 1091302
Detergent extraction of erythrocyte ghosts. Comparison of residues after cholate and Triton X-100 treatments.
Biochim Biophys Acta. 1976 Jun 4;436(1):38-44 PMID: 1276215
Characterization of membrane proteins in detergent solutions.
Biochim Biophys Acta. 1976 Oct 26;457(2):133-70 PMID: 135582
Effects of different bile salts upon the composition and morphology of a liver plasma membrane preparation. Deoxycholate is more membrane damaging than cholate and its conjugates.
Biochim Biophys Acta. 1977 Feb 14;465(1):68-76 PMID: 836833
Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants.
Anal Biochem. 1978 Jun 15;87(1):28-38 PMID: 677454
Properties of detergents.
Methods Enzymol. 1979;56:734-49 PMID: 459890
Electrofocusing of integral membrane proteins in mixtures of zwitterionic and nonionic detergents.
Anal Biochem. 1979 May;95(1):201-8 PMID: 495956
Solubilization of active opiate receptors.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623-7 PMID: 6254034

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.11.6368

Published

1980-11-00

Pages

6368-70

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350285

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.11.6368 PMC Full Text View journal details

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A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.

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